caa a22 4500 46789437X CHVBK 20180406152758.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s11103-006-0024-9 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0024-9 Heterologous Expression of a Mammalian ABC Transporter in Plant and its Application to Phytoremediation [Elektronische Daten] [Kazufumi Yazaki, Nao Yamanaka, Tsugumi Masuno, Satoshi Konagai, Shuji Kaneko, Kazumitsu Ueda, Fumihiko Sato] Mammalian ATP-binding cassette (ABC) transporters involved in the multidrug-resistance of cancer cells can efflux cytotoxic compounds that show a wide variety of chemical structures and biological activities. Human multidrug resistance-associated protein (hMRP1) is one of the most intensively studied ABC transporters and many substrates have been identified, including both organic and inorganic compounds. In an attempt at novel ‘transport engineering' using hMRP1 as a molecular pump, we established transgenic tobacco plants that showed clear resistance to cadmium and daunorubicin, although they were not resistant to etoposide, another known substrate of hMRP1. When expressed in tobacco cells, hMRP1 protein was localized at vacuolar membrane, while members of the MRP family are localized at plasma membrane in mammalian cells to reduce the cellular accumulation of various drugs. Thus, the hMRP1-expressing tobacco cells were able to take up these substrates across the tonoplast and sequestrate them in the vacuolar matrix. These results suggest that it may be possible to use the transgenic tobacco in phytoremediation, where a single transformation with an ABC transporter with broad substrate specificity should be effective for extracting various environmental pollutants including both organic and inorganic compounds, and accumulate them in the plant body. This should be advantageous for the remediation of a complex polluted environment, which is commonly found in the real world. Springer, 2006 ABC transporter nationallicence cadmium nationallicence complex pollutants nationallicence daunorubicin nationallicence MRP1 nationallicence phytoremediation nationallicence BSA : bovine serum albumin nationallicence DTT : dithiothreitol nationallicence EDTA : ethylenediamine tetraacetate nationallicence HEPES : N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid nationallicence MS : Murashige and Skoog nationallicence MRP : multidrug resistance-associated protein nationallicence PMSF : phenylmethylsulfonyl fluoride nationallicence PPase : pyrophosphatase nationallicence PVDF : polyvinylidene difluoride nationallicence PVPP : polyvinylpolypyrrolidone nationallicence YCF1 : yeast cadmium factor 1 nationallicence Yazaki Kazufumi Molecular & Cellular Biology of Totipotency, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kitashirakawa, Oiwake, 606-8502, Sakyo, Kyoto, Japan aut Yamanaka Nao Molecular & Cellular Biology of Totipotency, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kitashirakawa, Oiwake, 606-8502, Sakyo, Kyoto, Japan aut Masuno Tsugumi Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University, Gokaso, 611-0011, Uji, Kyoto, Japan aut Konagai Satoshi Molecular & Cellular Biology of Totipotency, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kitashirakawa, Oiwake, 606-8502, Sakyo, Kyoto, Japan aut Kaneko Shuji Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida-Shimo-Adachi, 606-8501, Sakyo, Kyoto, Japan aut Ueda Kazumitsu Laboratory of Cellular Biochemistry, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Oiwake, 606-8502, Sakyo, Kyoto, Japan aut Sato Fumihiko Molecular & Cellular Biology of Totipotency, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kitashirakawa, Oiwake, 606-8502, Sakyo, Kyoto, Japan aut Plant Molecular Biology Kluwer Academic Publishers 61/3(2006-06-01), 491-503 0167-4412 61:3<491 2006 61 11103 https://doi.org/10.1007/s11103-006-0024-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0024-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yazaki Kazufumi Molecular & Cellular Biology of Totipotency, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kitashirakawa, Oiwake, 606-8502, Sakyo, Kyoto, Japan aut NATIONALLICENCE 700 1- Yamanaka Nao Molecular & Cellular Biology of Totipotency, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kitashirakawa, Oiwake, 606-8502, Sakyo, Kyoto, Japan aut NATIONALLICENCE 700 1- Masuno Tsugumi Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University, Gokaso, 611-0011, Uji, Kyoto, Japan aut NATIONALLICENCE 700 1- Konagai Satoshi Molecular & Cellular Biology of Totipotency, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kitashirakawa, Oiwake, 606-8502, Sakyo, Kyoto, Japan aut NATIONALLICENCE 700 1- Kaneko Shuji Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida-Shimo-Adachi, 606-8501, Sakyo, Kyoto, Japan aut NATIONALLICENCE 700 1- Ueda Kazumitsu Laboratory of Cellular Biochemistry, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Oiwake, 606-8502, Sakyo, Kyoto, Japan aut NATIONALLICENCE 700 1- Sato Fumihiko Molecular & Cellular Biology of Totipotency, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kitashirakawa, Oiwake, 606-8502, Sakyo, Kyoto, Japan aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/3(2006-06-01), 491-503 0167-4412 61:3<491 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 467894264 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer