caa a22 4500 467894388 CHVBK 20180406152758.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s11103-006-0029-4 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0029-4 Evolution of Novel O -methyltransferases from the Vanilla planifolia Caffeic Acid O -methyltransferase [Elektronische Daten] [Huaijun Li, David Rotter, Thomas Hartman, Fulya Pak, Daphna Havkin-Frenkel, Faith Belanger] The biosynthesis of many plant secondary compounds involves the methylation of one or more hydroxyl groups, catalyzed by O-methyltransferases (OMTs). Here, we report the characterization of two OMTs, Van OMT-2 and Van OMT-3, from the orchid Vanilla planifolia Andrews. These enzymes catalyze the methylation of a single outer hydroxyl group in substrates possessing a 1,2,3-trihydroxybenzene moiety, such as methyl gallate and myricetin. This is a substrate requirement not previously reported for any OMTs. Based on sequence analysis these enzymes are most similar to caffeic acid O-methyltransferases (COMTs), but they have negligible activity with typical COMT substrates. Seven of 12 conserved substrate-binding residues in COMTs are altered in Van OMT-2 and Van OMT-3. Phylogenetic analysis of the sequences suggests that Van OMT-2 and Van OMT-3 evolved from the V. planifolia COMT. These V. planifolia OMTs are new instances of COMT-like enzymes with novel substrate preferences. Springer, 2006 O -methyltransferase nationallicence secondary metabolites nationallicence Vanilla nationallicence COMT : Caffeic acid O-methyltransferase nationallicence FOMT : flavonoid O-methyltransferase nationallicence IEMT : (iso)eugenol O-methyltransferase nationallicence IPTG : isopropyl-β-d-thiogalactopyranoside nationallicence OMT : O-methyltransferase nationallicence PCR : polymerase chain reaction nationallicence SAM : S-adenosyl-l-methionine nationallicence Li Huaijun Department of Plant Biology and Pathology and The Biotechnology Center for Agriculture & the Environment, Cook College, Rutgers University, 59 Dudley Road, 08901, New Brunswick, NJ, USA aut Rotter David Department of Plant Biology and Pathology and The Biotechnology Center for Agriculture & the Environment, Cook College, Rutgers University, 59 Dudley Road, 08901, New Brunswick, NJ, USA aut Hartman Thomas Center for Advanced Food Technology, Cook College, Rutgers University, 08901, New Brunswick, NJ, USA aut Pak Fulya Department of Plant Biology and Pathology and The Biotechnology Center for Agriculture & the Environment, Cook College, Rutgers University, 59 Dudley Road, 08901, New Brunswick, NJ, USA aut Havkin-Frenkel Daphna Department of Plant Biology and Pathology and The Biotechnology Center for Agriculture & the Environment, Cook College, Rutgers University, 59 Dudley Road, 08901, New Brunswick, NJ, USA aut Belanger Faith Department of Plant Biology and Pathology and The Biotechnology Center for Agriculture & the Environment, Cook College, Rutgers University, 59 Dudley Road, 08901, New Brunswick, NJ, USA aut Plant Molecular Biology Kluwer Academic Publishers 61/3(2006-06-01), 537-552 0167-4412 61:3<537 2006 61 11103 https://doi.org/10.1007/s11103-006-0029-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0029-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Huaijun Department of Plant Biology and Pathology and The Biotechnology Center for Agriculture & the Environment, Cook College, Rutgers University, 59 Dudley Road, 08901, New Brunswick, NJ, USA aut NATIONALLICENCE 700 1- Rotter David Department of Plant Biology and Pathology and The Biotechnology Center for Agriculture & the Environment, Cook College, Rutgers University, 59 Dudley Road, 08901, New Brunswick, NJ, USA aut NATIONALLICENCE 700 1- Hartman Thomas Center for Advanced Food Technology, Cook College, Rutgers University, 08901, New Brunswick, NJ, USA aut NATIONALLICENCE 700 1- Pak Fulya Department of Plant Biology and Pathology and The Biotechnology Center for Agriculture & the Environment, Cook College, Rutgers University, 59 Dudley Road, 08901, New Brunswick, NJ, USA aut NATIONALLICENCE 700 1- Havkin-Frenkel Daphna Department of Plant Biology and Pathology and The Biotechnology Center for Agriculture & the Environment, Cook College, Rutgers University, 59 Dudley Road, 08901, New Brunswick, NJ, USA aut NATIONALLICENCE 700 1- Belanger Faith Department of Plant Biology and Pathology and The Biotechnology Center for Agriculture & the Environment, Cook College, Rutgers University, 59 Dudley Road, 08901, New Brunswick, NJ, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/3(2006-06-01), 537-552 0167-4412 61:3<537 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer