caa a22 4500 46789440X CHVBK 20180406152758.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s11103-006-0023-x doi (NATIONALLICENCE)springer-10.1007/s11103-006-0023-x A Homeodomain Leucine Zipper Gene from Craterostigma plantagineum Regulates Abscisic Acid Responsive Gene Expression and Physiological Responses [Elektronische Daten] [Xin Deng, Jonathan Phillips, Anne Bräutigam, Peter Engström, Henrik Johannesson, Pieter Ouwerkerk, Ida Ruberti, Julio Salinas, Pablo Vera, Rina Iannacone, Annemarie Meijer, Dorothea Bartels] A subset of homeodomain leucine zipper proteins (HDZip) play a role in regulating adaptation responses including developmental adjustment to environmental cues in plants. Here we report the structural and functional characterisation of a dehydration responsive nuclear-targeted HDZip transcriptional regulator, CpHB-7. DNA-protein interaction studies suggest that CDeT6-19, a known ABA and dehydration responsive dehydrin gene, is a potential target gene of CpHB-7 in the desiccation-tolerant plant Craterostigma plantagineum. Transgenic plants that ectopically express CpHB-7 display reduced sensitivity towards ABA during seed germination and stomatal closure. Expression analysis reveals that genes with induced or repressed expression in CpHB-7 ectopic expression lines are either mostly repressed or induced by ABA, drought or salt treatment respectively, thus demonstrating that CpHB-7 modifies ABA-responsive gene expression as a negative regulator. CpHB-7 gene expression is also linked to early organ development, leading to the suggestion that CpHB-7 is functionally similar to the Arabidopsis transcription factor, ATHB-6. Springer, 2006 abscisic acid nationallicence Craterostigma plantagineum nationallicence dehydration tolerance nationallicence homeodomain leucine zipper protein nationallicence Deng Xin Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, D-50829, Cologne, Germany aut Phillips Jonathan Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, D-50829, Cologne, Germany aut Bräutigam Anne Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, D-50829, Cologne, Germany aut Engström Peter Department of Physiological Botany, Uppsala University, Villavaegen 6, 752 36, Uppsala, Sweden aut Johannesson Henrik Department of Physiological Botany, Uppsala University, Villavaegen 6, 752 36, Uppsala, Sweden aut Ouwerkerk Pieter Institute of Biology, Leiden University, Wassenaarseweg 64, 2333, Rome, AL, The Netherlands aut Ruberti Ida Institute of Molecular Biology and Pathology National Research Council, P.le Aldo Moro 5, 00185, Italy aut Salinas Julio Departamento de Biotecnologia, Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA), Carretera de la Coruna Km. 7, 28040, Madrid, Spain aut Vera Pablo Instituto de Biología Molecular y Celular de Plantas (IBMCP) UPV-CSIC, Camino de Vera s/n, 46022, Valencia, Spain aut Iannacone Rina Metapontum Agrobios s.r.l, S.S. Jonica 106 Km 448,2, 75010, Metaponto, Italy aut Meijer Annemarie Institute of Biology, Leiden University, Wassenaarseweg 64, 2333, Rome, AL, The Netherlands aut Bartels Dorothea Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, D-50829, Cologne, Germany aut Plant Molecular Biology Kluwer Academic Publishers 61/3(2006-06-01), 469-489 0167-4412 61:3<469 2006 61 11103 https://doi.org/10.1007/s11103-006-0023-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0023-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Deng Xin Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, D-50829, Cologne, Germany aut NATIONALLICENCE 700 1- Phillips Jonathan Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, D-50829, Cologne, Germany aut NATIONALLICENCE 700 1- Bräutigam Anne Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, D-50829, Cologne, Germany aut NATIONALLICENCE 700 1- Engström Peter Department of Physiological Botany, Uppsala University, Villavaegen 6, 752 36, Uppsala, Sweden aut NATIONALLICENCE 700 1- Johannesson Henrik Department of Physiological Botany, Uppsala University, Villavaegen 6, 752 36, Uppsala, Sweden aut NATIONALLICENCE 700 1- Ouwerkerk Pieter Institute of Biology, Leiden University, Wassenaarseweg 64, 2333, Rome, AL, The Netherlands aut NATIONALLICENCE 700 1- Ruberti Ida Institute of Molecular Biology and Pathology National Research Council, P.le Aldo Moro 5, 00185, Italy aut NATIONALLICENCE 700 1- Salinas Julio Departamento de Biotecnologia, Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA), Carretera de la Coruna Km. 7, 28040, Madrid, Spain aut NATIONALLICENCE 700 1- Vera Pablo Instituto de Biología Molecular y Celular de Plantas (IBMCP) UPV-CSIC, Camino de Vera s/n, 46022, Valencia, Spain aut NATIONALLICENCE 700 1- Iannacone Rina Metapontum Agrobios s.r.l, S.S. Jonica 106 Km 448,2, 75010, Metaponto, Italy aut NATIONALLICENCE 700 1- Meijer Annemarie Institute of Biology, Leiden University, Wassenaarseweg 64, 2333, Rome, AL, The Netherlands aut NATIONALLICENCE 700 1- Bartels Dorothea Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, D-50829, Cologne, Germany aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/3(2006-06-01), 469-489 0167-4412 61:3<469 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer