caa a22 4500 467894418 CHVBK 20180406152758.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s11103-006-0019-6 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0019-6 Disease Resistance in Plants that Carry a Feedback-regulated Yeast Poly(A) Binding Protein Gene [Elektronische Daten] [Balasubrahmanyam Addepalli, Ruqiang Xu, Tomal Dattaroy, Baochun Li, W. Bass, Qingshun Li, Arthur Hunt] It has been reported that the expression of the yeast poly(A) binding protein gene (PAB1) in plants leads to an induction of disease resistance responses, accompanied by alterations in the growth habit of the plant (Li et al. Plant Mol. Biol. (2000) 42 335). To capitalize on this observation, a feedback-regulated PAB1 gene was assembled and introduced into tobacco and Arabidopsis. The regulation entailed the linking of the expression of the PAB1 gene to control by the lac repressor, and by linking lac repressor expression to the disease resistance state of the plant, such that the induction of systemic defense responses by accumulation of the yeast poly(A) binding protein would turn off the expression of the PAB1 gene. Plants containing this system showed elevated and/or constitutive expression of disease-associated genes and significant resistance to otherwise pathogenic organisms. As well, they displayed a nearly normal growth habit under laboratory and greenhouse settings. These studies indicate that the expression of cytotoxic genes (such as the PAB1 gene) in plants can be controlled so that enhanced disease resistance can be achieved without significantly affecting plant growth and development. Springer, 2006 constitutive PR gene expression nationallicence disease resistance nationallicence feedback regulation nationallicence lac repressor nationallicence yeast poly(A) binding protein nationallicence Addepalli Balasubrahmanyam Department of Plant and Soil Sciences, University of Kentucky, 40546-0312, Lexington, KY, USA aut Xu Ruqiang Department of Botany, Miami University, 45056, Oxford, OH, USA aut Dattaroy Tomal Department of Plant and Soil Sciences, University of Kentucky, 40546-0312, Lexington, KY, USA aut Li Baochun Kentucky Tobacco Research and Development Center, University of Kentucky, Cooper & University Drives, 40546-0236, Lexington, KY, USA aut Bass W. Kentucky Tobacco Research and Development Center, University of Kentucky, Cooper & University Drives, 40546-0236, Lexington, KY, USA aut Li Qingshun Department of Botany, Miami University, 45056, Oxford, OH, USA aut Hunt Arthur Department of Plant and Soil Sciences, University of Kentucky, 40546-0312, Lexington, KY, USA aut Plant Molecular Biology Kluwer Academic Publishers 61/3(2006-06-01), 383-397 0167-4412 61:3<383 2006 61 11103 https://doi.org/10.1007/s11103-006-0019-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0019-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Addepalli Balasubrahmanyam Department of Plant and Soil Sciences, University of Kentucky, 40546-0312, Lexington, KY, USA aut NATIONALLICENCE 700 1- Xu Ruqiang Department of Botany, Miami University, 45056, Oxford, OH, USA aut NATIONALLICENCE 700 1- Dattaroy Tomal Department of Plant and Soil Sciences, University of Kentucky, 40546-0312, Lexington, KY, USA aut NATIONALLICENCE 700 1- Li Baochun Kentucky Tobacco Research and Development Center, University of Kentucky, Cooper & University Drives, 40546-0236, Lexington, KY, USA aut NATIONALLICENCE 700 1- Bass W. Kentucky Tobacco Research and Development Center, University of Kentucky, Cooper & University Drives, 40546-0236, Lexington, KY, USA aut NATIONALLICENCE 700 1- Li Qingshun Department of Botany, Miami University, 45056, Oxford, OH, USA aut NATIONALLICENCE 700 1- Hunt Arthur Department of Plant and Soil Sciences, University of Kentucky, 40546-0312, Lexington, KY, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/3(2006-06-01), 383-397 0167-4412 61:3<383 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer