caa a22 4500 467894469 CHVBK 20180406152758.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s11103-006-0020-0 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0020-0 Silencing of an Anther-specific Zinc-finger Gene, MEZ1 , Causes Aberrant Meiosis and Pollen Abortion in Petunia [Elektronische Daten] [Sanjay Kapoor, Hiroshi Takatsuji] MEZ1 (MEiosis-associated Zinc-finger protein 1) was first isolated as an anther-specific cDNA from Petunia hybrida. In the present study, we report its functional characterization, including its spatial and temporal expression profiles and phenotypes in MEZ1-silenced plants. MEZ1 transcripts were specifically localized in pollen mother cells during early stages of anther development, and were later distributed in vegetative tissues in anthers. Silencing of MEZ1 by cosuppression resulted in several anomalies during male meiosis that included inability of chromosomes to condense, loss of meiotic synchrony, and premature and apparently uncontrolled cytokinetic events. Consequently, by the end of meiosis 8-10 cells, instead of the normal 4, with varying DNA contents were formed in the MEZ1-silenced meiocytes. Most of these aborted prematurely, and those that matured had a distinctive morphology. MEZ1-silenced plants were female sterile when pollinated with wild-type pollen but they infrequently produced a few seeds upon self-pollination. Resulting T1 plants had increased ploidy levels and exhibited severe anomalies during male meiosis, rendering them completely sterile. We discuss possible role of MEZ1 in the proper progression of plant meiosis. Springer, 2006 male sterile nationallicence meiosis nationallicence petunia nationallicence ploidy nationallicence transcription factor nationallicence zinc-finger nationallicence am1 : ameiotic1 nationallicence Bl : bud length nationallicence CaMV : cauliflower mosaic virus nationallicence DAP : days after pollination nationallicence DAPI : 4′,6-diamidino-2-phenylindole nationallicence MEZ1 : MEiosis-associated Zinc-finger protein 1 nationallicence cMEZ1 : MEZ1 cDNA nationallicence NLS : nuclear localization signal nationallicence PMC : pollen mother cell nationallicence SAP : STERILE APETALA nationallicence SPL/NZZ : SPOROCYTELESS/NOZZLE nationallicence SWI1 : Switch1 nationallicence Kapoor Sanjay Developmental Biology Laboratory, Plant Physiology Department, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Ibaraki, Japan aut Takatsuji Hiroshi Developmental Biology Laboratory, Plant Physiology Department, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Ibaraki, Japan aut Plant Molecular Biology Kluwer Academic Publishers 61/3(2006-06-01), 415-430 0167-4412 61:3<415 2006 61 11103 https://doi.org/10.1007/s11103-006-0020-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0020-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kapoor Sanjay Developmental Biology Laboratory, Plant Physiology Department, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Takatsuji Hiroshi Developmental Biology Laboratory, Plant Physiology Department, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/3(2006-06-01), 415-430 0167-4412 61:3<415 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer