caa a22 4500 467894477 CHVBK 20180406152758.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s11103-006-0022-y doi (NATIONALLICENCE)springer-10.1007/s11103-006-0022-y ARL1 Plays a Role in the Binding of the GRIP Domain of a Peripheral Matrix Protein to the Golgi Apparatus in Plant Cells [Elektronische Daten] [Giovanni Stefano, Luciana Renna, Sally Hanton, Laurent Chatre, Thomas Haas, Federica Brandizzi] ARF GTPases play a central role in regulating membrane dynamics and protein transport in eukaryotic cells. ARF-like (ARL) proteins are close relatives of the ARF regulators of vesicular transport, but their function in plant cells is poorly characterized. Here, by means of live cell imaging and site-directed mutagenesis, we have investigated the cellular function of the plant GTPase ARL1. We provide direct evidence for a role of this ARL family member in the association of a plant golgin with the plant Golgi apparatus. Our data reveal the existence of key residues within the conserved GRIP-domain of the golgin and within the GTPase ARL1 that are central to ARL1-GRIP interaction. Mutations of these residues abolish the interaction of GRIP with the GTP-bound ARL1 and induce a redistribution of GRIP into the cytosol. This indicates that the localization of GRIP to the Golgi apparatus is strongly influenced by the interaction of GRIP with Golgi-localized ARL1. Our results assign a cellular role to a member of the Arabidopsis ARL family in the plant secretory pathway and propose mechanisms for localization of peripheral golgins to the plant Golgi apparatus. Springer, 2006 ARF-like proteins nationallicence Golgi apparatus nationallicence Golgins nationallicence Stefano Giovanni Department of Biology, University of Saskatchewan, 112 Science Place, S7N 5E2, Saskatoon, SK, Canada aut Renna Luciana Department of Biology, University of Saskatchewan, 112 Science Place, S7N 5E2, Saskatoon, SK, Canada aut Hanton Sally Department of Biology, University of Saskatchewan, 112 Science Place, S7N 5E2, Saskatoon, SK, Canada aut Chatre Laurent Department of Biology, University of Saskatchewan, 112 Science Place, S7N 5E2, Saskatoon, SK, Canada aut Haas Thomas Department of Anatomy and Cell Biology, University of Saskatchewan, 107 Wiggins Road, S7N 5E5, Saskatoon, SK, Canada aut Brandizzi Federica Department of Biology, University of Saskatchewan, 112 Science Place, S7N 5E2, Saskatoon, SK, Canada aut Plant Molecular Biology Kluwer Academic Publishers 61/3(2006-06-01), 431-449 0167-4412 61:3<431 2006 61 11103 https://doi.org/10.1007/s11103-006-0022-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0022-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stefano Giovanni Department of Biology, University of Saskatchewan, 112 Science Place, S7N 5E2, Saskatoon, SK, Canada aut NATIONALLICENCE 700 1- Renna Luciana Department of Biology, University of Saskatchewan, 112 Science Place, S7N 5E2, Saskatoon, SK, Canada aut NATIONALLICENCE 700 1- Hanton Sally Department of Biology, University of Saskatchewan, 112 Science Place, S7N 5E2, Saskatoon, SK, Canada aut NATIONALLICENCE 700 1- Chatre Laurent Department of Biology, University of Saskatchewan, 112 Science Place, S7N 5E2, Saskatoon, SK, Canada aut NATIONALLICENCE 700 1- Haas Thomas Department of Anatomy and Cell Biology, University of Saskatchewan, 107 Wiggins Road, S7N 5E5, Saskatoon, SK, Canada aut NATIONALLICENCE 700 1- Brandizzi Federica Department of Biology, University of Saskatchewan, 112 Science Place, S7N 5E2, Saskatoon, SK, Canada aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/3(2006-06-01), 431-449 0167-4412 61:3<431 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer