caa a22 4500 467894507 CHVBK 20180406152759.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s11103-005-5966-9 doi (NATIONALLICENCE)springer-10.1007/s11103-005-5966-9 Spatial Organisation of Four Enzymes from Stevia rebaudiana that are Involved in Steviol Glycoside Synthesis [Elektronische Daten] [Tania Humphrey, Alex Richman, Rima Menassa, Jim Brandle] The sweet steviol glycosides found in the leaves of Stevia rebaudiana Bert. are derived from the diterpene steviol which is produced from a branch of the gibberellic acid (GA) biosynthetic pathway. An understanding of the spatial organisation of the two pathways including subcellular compartmentation provides important insight for the metabolic engineering of steviol glycosides as well as other secondary metabolites in plants. The final step of GA biosynthesis, before the branch point for steviol production, is the formation of (−)-kaurenoic acid from (−)-kaurene, catalysed by kaurene oxidase (KO). Downstream of this, the first committed step in steviol glycoside synthesis is the hydroxylation of kaurenoic acid to form steviol which is then sequentially glucosylated by a series of UDP-glucosyltransferases (UGTs) to produce the variety of steviol glycosides. The subcellular location of KO and three of the UGTs involved in steviol glycoside biosynthesis was investigated by expression of GFP fusions and cell fractionation which revealed KO to be associated with the endoplasmic reticulum and the UGTs in the cytoplasm. It has also been shown by expressing the Stevia UGTs in Arabidopsis that the pathway can be partially reconstituted by recruitment of a native Arabidopsis glucosyltransferase. Springer, 2006 cytochrome P450 nationallicence diterpene nationallicence gibberellin nationallicence kaurene oxidase nationallicence metabolic engineering nationallicence UDP-glucosyltransferase nationallicence Humphrey Tania Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, 1391 Sandford St., N5V 4T3, London, Ont., Canada aut Richman Alex Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, 1391 Sandford St., N5V 4T3, London, Ont., Canada aut Menassa Rima Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, 1391 Sandford St., N5V 4T3, London, Ont., Canada aut Brandle Jim Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, 1391 Sandford St., N5V 4T3, London, Ont., Canada aut Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 47-62 0167-4412 61:1-2<47 2006 61 11103 https://doi.org/10.1007/s11103-005-5966-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-5966-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Humphrey Tania Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, 1391 Sandford St., N5V 4T3, London, Ont., Canada aut NATIONALLICENCE 700 1- Richman Alex Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, 1391 Sandford St., N5V 4T3, London, Ont., Canada aut NATIONALLICENCE 700 1- Menassa Rima Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, 1391 Sandford St., N5V 4T3, London, Ont., Canada aut NATIONALLICENCE 700 1- Brandle Jim Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, 1391 Sandford St., N5V 4T3, London, Ont., Canada aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 47-62 0167-4412 61:1-2<47 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer