caa a22 4500 467894515 CHVBK 20180406152759.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s11103-006-0015-x doi (NATIONALLICENCE)springer-10.1007/s11103-006-0015-x Subtractive cDNA Libraries Identify Differentially Expressed Genes in Dormant and Growing Buds of Leafy Spurge ( Euphorbia esula ) [Elektronische Daten] [Ying Jia, James Anderson, David Horvath, Yong-Qiang Gu, Rodney Lym, Wun Chao] Two subtractive cDNA libraries were developed to study genes associated with bud dormancy (reverse library) and initiation of shoot growth (forward library) in leafy spurge. To identify unique sequences represented in each library, 15744 clones were screened to reduce the level of redundancy within both libraries. A total of 516 unique sequences were obtained from 2304 minimally redundant clones. Radioactive probes developed from RNAs extracted from crown buds of either intact (para-dormant control) or a series of growth-induced (2h, 2, and 4d after decapitation) plants were used to identify differentially expressed genes by macroarray analysis. Semi-quantitative RT-PCR was used to confirm results obtained by macroarray analysis and to determine the expression profiles for other transcripts identified within the subtractive libraries. Selected clones were also used to examine gene expression in crown buds after growth induction and/or during normal seasonal growth. In this study, four distinct patterns of gene expression were observed during the transition from para-dormancy to growth-induction. Many of the differentially regulated genes identified have unknown or hypothetical functions while others are known to play important roles in molecular functions. Gene ontology analysis identified a greater proportion of genes involved with catalytic activity in the forward library while the reverse library had a greater proportion of genes involved in DNA/RNA binding. Springer, 2006 dormancy nationallicence leafy spurge nationallicence subtractive hybridization nationallicence Jia Ying Department of Plant Sciences, North Dakota State University, 58105, Fargo, ND, USA aut Anderson James Plant Science Research, USDA-Agricultural Research Service, 1605 Albrecht Blvd., 58105-5674, Fargo, ND, USA aut Horvath David Plant Science Research, USDA-Agricultural Research Service, 1605 Albrecht Blvd., 58105-5674, Fargo, ND, USA aut Gu Yong-Qiang Western Regional Research Center, USDA-ARS, 94710, Albany, CA, USA aut Lym Rodney Department of Plant Sciences, North Dakota State University, 58105, Fargo, ND, USA aut Chao Wun Plant Science Research, USDA-Agricultural Research Service, 1605 Albrecht Blvd., 58105-5674, Fargo, ND, USA aut Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 329-344 0167-4412 61:1-2<329 2006 61 11103 https://doi.org/10.1007/s11103-006-0015-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0015-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jia Ying Department of Plant Sciences, North Dakota State University, 58105, Fargo, ND, USA aut NATIONALLICENCE 700 1- Anderson James Plant Science Research, USDA-Agricultural Research Service, 1605 Albrecht Blvd., 58105-5674, Fargo, ND, USA aut NATIONALLICENCE 700 1- Horvath David Plant Science Research, USDA-Agricultural Research Service, 1605 Albrecht Blvd., 58105-5674, Fargo, ND, USA aut NATIONALLICENCE 700 1- Gu Yong-Qiang Western Regional Research Center, USDA-ARS, 94710, Albany, CA, USA aut NATIONALLICENCE 700 1- Lym Rodney Department of Plant Sciences, North Dakota State University, 58105, Fargo, ND, USA aut NATIONALLICENCE 700 1- Chao Wun Plant Science Research, USDA-Agricultural Research Service, 1605 Albrecht Blvd., 58105-5674, Fargo, ND, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 329-344 0167-4412 61:1-2<329 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer