caa a22 4500 467894523 CHVBK 20180406152759.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s11103-006-0016-9 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0016-9 CENTRIN2 Interacts with the Arabidopsis Homolog of the Human XPC Protein (AtRAD4) and Contributes to Efficient Synthesis-dependent Repair of Bulky DNA Lesions [Elektronische Daten] [Lu Liang, Sabine Flury, Véronique Kalck, Barbara Hohn, Jean Molinier] Arabidopsis thaliana CENTRIN2 (AtCEN2) has been shown to modulate Nucleotide Excision Repair (NER) and Homologous Recombination (HR). The present study provides evidence that AtCEN2 interacts with the Arabidopsis homolog of human XPC, AtRAD4 and that the distal EF-hand Ca2+ binding domain is essential for this interaction. In addition, the synthesis-dependent repair efficiency of bulky DNA lesions was enhanced in cell extracts prepared from Arabidopsis plants overexpressing the full length AtCEN2 but not in those overexpressing a truncated AtCEN2 form, suggesting a role for the distal EF-hand Ca2+ binding domain in the early step of the NER process. Upon UV-C treatment the AtCEN2 protein was shown to be increased in concentration and to be localised in the nucleus rapidly. Taken together these data suggest that AtCEN2 is a part of the AtRAD4 recognition complex and that this interaction is required for efficient NER. In addition, NER and HR appear to be differentially modulated upon exposure of plants to DNA damaging agents. This suggests in plants, that processing of bulky DNA lesions highly depends on the excision repair efficiency, especially the recognition step, thus influencing the recombinational repair pathway. Springer, 2006 centrin nationallicence homologous recombination nationallicence nucleotide excision repair nationallicence Rad4 nationallicence BER : Base Excision Repair nationallicence DSB : Double Strand Break nationallicence GFP : Green Fluorescent Protein nationallicence HR : Homologous Recombination nationallicence ICL : Inter/Intra Crosslink nationallicence NHEJ : Non Homologous End Joining nationallicence NER : Nucleotide Excision Repair nationallicence MMR : Mismatch Repair nationallicence XP : Xeroderma Pigmentosum nationallicence Liang Lu Plant Biochemistry Physiology Group, Federal Institute of Technology (ETH) Zurich, Institute of Plant Sciences, Experimental Station Eschikon 33, CH-8315, Lindau, Switzerland aut Flury Sabine Swiss Federal Institute for Environmental Science & Technology (EAWAG), Limnogical Research, Ueberlandstrasse 133, CH-8600, Duebendorf, Switzerland aut Kalck Véronique Friedrich Miescher Institute, Maulbeerstrasse 66, CH 4058, Basel, Switzerland aut Hohn Barbara Friedrich Miescher Institute, Maulbeerstrasse 66, CH 4058, Basel, Switzerland aut Molinier Jean Friedrich Miescher Institute, Maulbeerstrasse 66, CH 4058, Basel, Switzerland aut Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 345-356 0167-4412 61:1-2<345 2006 61 11103 https://doi.org/10.1007/s11103-006-0016-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0016-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liang Lu Plant Biochemistry Physiology Group, Federal Institute of Technology (ETH) Zurich, Institute of Plant Sciences, Experimental Station Eschikon 33, CH-8315, Lindau, Switzerland aut NATIONALLICENCE 700 1- Flury Sabine Swiss Federal Institute for Environmental Science & Technology (EAWAG), Limnogical Research, Ueberlandstrasse 133, CH-8600, Duebendorf, Switzerland aut NATIONALLICENCE 700 1- Kalck Véronique Friedrich Miescher Institute, Maulbeerstrasse 66, CH 4058, Basel, Switzerland aut NATIONALLICENCE 700 1- Hohn Barbara Friedrich Miescher Institute, Maulbeerstrasse 66, CH 4058, Basel, Switzerland aut NATIONALLICENCE 700 1- Molinier Jean Friedrich Miescher Institute, Maulbeerstrasse 66, CH 4058, Basel, Switzerland aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 345-356 0167-4412 61:1-2<345 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer