caa a22 4500 467894531 CHVBK 20180406152759.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s11103-006-6408-z doi (NATIONALLICENCE)springer-10.1007/s11103-006-6408-z Characterization of T-DNA Insertion Mutants and RNAi Silenced Plants of Arabidopsis thaliana UV-damaged DNA Binding Protein 2 ( AtUV-DDB2 ) [Elektronische Daten] [Asami Koga, Toyotaka Ishibashi, Seisuke Kimura, Yukinobu Uchiyama, Kengo Sakaguchi] The human UV-damaged DNA binding protein (UV-DDB), a heterodimeric protein composed of 127kDa (UV-DDB1) and 48kDa (UV-DDB2) subunits, has been shown to be involved in DNA repair. To elucidate the in vivo function of plant UV-DDB2, we have analyzed T-DNA insertion mutants of the Arabidopsis thaliana UV-DDB2 subunit (atuv-ddb2 mutants) and AtUV-DDB2 RNAi silenced plants (atuv-ddb2 silenced plants). atuv-ddb2 mutants and atuv-ddb2 silenced plants were both viable, suggesting that AtUV-DDB2 is not essential for survival. Interestingly, both plant types showed a dwarf phenotype, implying impaired growth of the meristem. To the best of our knowledge, this is the first occasion that a dwarf phenotype has been found to be associated with a UV-DDB2 mutation in either plants or animals. The mutants also demonstrated increased sensitivity to UV irradiation, methyl methanesulfonate and hydrogen peroxide treatment, indicating that AtUV-DDB2 is also involved in DNA repair. Our results lead us to suggest that not only does AtUV-DDB2 function in DNA repair, it also has a direct or indirect influence on cell proliferation in the plant meristem. Springer, 2006 UV-damaged DNA binding protein complex (UV-DDB) nationallicence Arabidopsis thaliana nationallicence T-DNA insertion line nationallicence gene silencing by RNAi nationallicence DNA repair nationallicence Koga Asami Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda, Chiba, Japan aut Ishibashi Toyotaka Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda, Chiba, Japan aut Kimura Seisuke Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda, Chiba, Japan aut Uchiyama Yukinobu Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda, Chiba, Japan aut Sakaguchi Kengo Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda, Chiba, Japan aut Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 227-240 0167-4412 61:1-2<227 2006 61 11103 https://doi.org/10.1007/s11103-006-6408-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-6408-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koga Asami Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda, Chiba, Japan aut NATIONALLICENCE 700 1- Ishibashi Toyotaka Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda, Chiba, Japan aut NATIONALLICENCE 700 1- Kimura Seisuke Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda, Chiba, Japan aut NATIONALLICENCE 700 1- Uchiyama Yukinobu Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda, Chiba, Japan aut NATIONALLICENCE 700 1- Sakaguchi Kengo Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda, Chiba, Japan aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 227-240 0167-4412 61:1-2<227 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer