caa a22 4500 46789454X CHVBK 20180406152759.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s11103-006-6265-9 doi (NATIONALLICENCE)springer-10.1007/s11103-006-6265-9 RNA Interference of the Arabidopsis Putative Transcription Factor TCP16 Gene Results in Abortion of Early Pollen Development [Elektronische Daten] [Taito Takeda, Kazuo Amano, Masa-aki Ohto, Kenzo Nakamura, Shusei Sato, Tomohiko Kato, Satoshi Tabata, Chiharu Ueguchi] Pollen development is a fundamental and essential biological process in seed plants. Pollen mother cells generated in anthers undergo meiosis, which gives rise to haploid microspores. The haploid cells then develop into mature pollen grains through two mitotic cell divisions. Although several sporophytic and gametophytic mutations affecting male gametogenesis have been identified and analyzed, little is known about the underlying molecular mechanism. In this study, we investigated the function of the TCP16 gene, which encodes a putative transcription factor. Expression analysis of the promoter::GUS fusion gene revealed that TCP16 transcription occurred predominantly in developing microspores. GUS expression began at the tetrad stage and markedly increased in an early unicellular stage. Transgenic plants harboring a TCP16 RNA interference (RNAi) construct generated equal amounts of normal and abnormal pollen grains. The abnormal pollen grains exhibited morphological abnormality and degeneration of genomic DNA. The defective phenotype of the RNAi plants was first detectable at the middle of the unicellular stage. Our results therefore suggest that TCP16, a putative transcription factor, plays a crucial role in early processes in pollen development. Springer, 2006 pollen development nationallicence RNA interference nationallicence TCP domain nationallicence transcription factor nationallicence Takeda Taito Bioscience and Biotechnology Center, Nagoya University, 464-8601, Chikusa-ku, Nagoya, Japan aut Amano Kazuo Bioscience and Biotechnology Center, Nagoya University, 464-8601, Chikusa-ku, Nagoya, Japan aut Ohto Masa-aki Division of Developmental Biology, National Institute for Basic Biology, 444-8585, Myodaiji-cho, Okazaki, Japan aut Nakamura Kenzo Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Chikusa-ku, Nagoya, Japan aut Sato Shusei Kazusa DNA Research Institute, 292-0812, Kisarazu, Japan aut Kato Tomohiko Kazusa DNA Research Institute, 292-0812, Kisarazu, Japan aut Tabata Satoshi Kazusa DNA Research Institute, 292-0812, Kisarazu, Japan aut Ueguchi Chiharu Bioscience and Biotechnology Center, Nagoya University, 464-8601, Chikusa-ku, Nagoya, Japan aut Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 165-177 0167-4412 61:1-2<165 2006 61 11103 https://doi.org/10.1007/s11103-006-6265-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-6265-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takeda Taito Bioscience and Biotechnology Center, Nagoya University, 464-8601, Chikusa-ku, Nagoya, Japan aut NATIONALLICENCE 700 1- Amano Kazuo Bioscience and Biotechnology Center, Nagoya University, 464-8601, Chikusa-ku, Nagoya, Japan aut NATIONALLICENCE 700 1- Ohto Masa-aki Division of Developmental Biology, National Institute for Basic Biology, 444-8585, Myodaiji-cho, Okazaki, Japan aut NATIONALLICENCE 700 1- Nakamura Kenzo Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Chikusa-ku, Nagoya, Japan aut NATIONALLICENCE 700 1- Sato Shusei Kazusa DNA Research Institute, 292-0812, Kisarazu, Japan aut NATIONALLICENCE 700 1- Kato Tomohiko Kazusa DNA Research Institute, 292-0812, Kisarazu, Japan aut NATIONALLICENCE 700 1- Tabata Satoshi Kazusa DNA Research Institute, 292-0812, Kisarazu, Japan aut NATIONALLICENCE 700 1- Ueguchi Chiharu Bioscience and Biotechnology Center, Nagoya University, 464-8601, Chikusa-ku, Nagoya, Japan aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 165-177 0167-4412 61:1-2<165 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer