caa a22 4500 467894558 CHVBK 20180406152759.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s11103-006-0008-9 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0008-9 Towards Positional Cloning in Brassica napus [Elektronische Daten] Generation and Analysis of Doubled Haploid B. rapa Possessing the B. napus pol CMS and Rfp Nuclear Restorer Gene [Nataša Formanová, Xiu-Qing Li, Alison Ferrie, Mary DePauw, Wilf Keller, Benoit Landry, Gregory Brown] The Polima (pol) system of cytoplasmic male sterility (CMS) and its fertility restorer gene Rfp are used in hybrid rapeseed production in Brassica napus. To facilitate map-based cloning of the Rfp gene, we have successfully transferred the pol cytoplasm and Rfp from the amphidiploid B. napus to the diploid species B. rapa and generated a doubled haploid pol cytoplasm B. rapa population that segregates for the Rfp gene. This was achieved through interspecific crosses, in vitro rescue of hybrid embryos, backcrosses, and microspore culture. Male fertility conditioned by Rfp was shown to co-segregate in this population with Rfp-specific mitochondrial transcript modifications and with DNA markers previously shown to be linked to Rfp in B. napus. The selfed-progeny of one doubled haploid plant were confirmed to be characteristic B. rapa diploids by cytogenetic analysis. Clones recovered from a genomic library derived from this plant line using the RFLP probe cRF1 fell into several distinct physical contigs, one of which contained Rfp-linked polymorphic restriction fragments detected by this probe. This indicates that chromosomal DNA segments anchored in the Rfp region can be recovered from this library and that the library may therefore prove to be a useful resource for the eventual isolation of the Rfp gene. Springer, 2006 comparative genomics nationallicence homeology nationallicence mitochondrial gene expression nationallicence hybrid seed nationallicence canola nationallicence genetic mapping nationallicence doubled haploid nationallicence RNA processing nationallicence CMS : cytoplasmic male sterility nationallicence DH : doubled haploid nationallicence h : hour nationallicence min : minute nationallicence kb : kilobase nationallicence PPR : pentatricopeptide nationallicence Rf : restorer of fertility nationallicence Formanová Nataša Department of Biology, McGill University, H3A 1B1, Montreal, Quebec, Canada aut Li Xiu-Qing Department of Biology, McGill University, H3A 1B1, Montreal, Quebec, Canada aut Ferrie Alison Plant Biotechnology Institute, National Research Counci, 110 Gymnasium Place, S7N OW9, Saskatoon, Saskatchewan, Canada aut DePauw Mary Department of Biology, McGill University, H3A 1B1, Montreal, Quebec, Canada aut Keller Wilf Plant Biotechnology Institute, National Research Counci, 110 Gymnasium Place, S7N OW9, Saskatoon, Saskatchewan, Canada aut Landry Benoit DNA LandMarks Inc., 84 rue Richelieu, St-Jean-sur-Richelieu, Quebec, Canada aut Brown Gregory Department of Biology, McGill University, H3A 1B1, Montreal, Quebec, Canada aut Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 269-281 0167-4412 61:1-2<269 2006 61 11103 https://doi.org/10.1007/s11103-006-0008-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0008-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Formanová Nataša Department of Biology, McGill University, H3A 1B1, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Li Xiu-Qing Department of Biology, McGill University, H3A 1B1, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Ferrie Alison Plant Biotechnology Institute, National Research Counci, 110 Gymnasium Place, S7N OW9, Saskatoon, Saskatchewan, Canada aut NATIONALLICENCE 700 1- DePauw Mary Department of Biology, McGill University, H3A 1B1, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Keller Wilf Plant Biotechnology Institute, National Research Counci, 110 Gymnasium Place, S7N OW9, Saskatoon, Saskatchewan, Canada aut NATIONALLICENCE 700 1- Landry Benoit DNA LandMarks Inc., 84 rue Richelieu, St-Jean-sur-Richelieu, Quebec, Canada aut NATIONALLICENCE 700 1- Brown Gregory Department of Biology, McGill University, H3A 1B1, Montreal, Quebec, Canada aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 269-281 0167-4412 61:1-2<269 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer