caa a22 4500 467894574 CHVBK 20180406152759.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s11103-006-0005-z doi (NATIONALLICENCE)springer-10.1007/s11103-006-0005-z Auxin Flow in Anther Filaments is Critical for Pollen Grain Development through Regulating Pollen Mitosis [Elektronische Daten] [Xiao-Li Feng, Wei-Min Ni, Stephan Elge, Bernd Mueller-Roeber, Zhi-Hong Xu, Hong-Wei Xue] It was well known that auxin is critical for anther/pollen grain development, however, the clear distribution and detailed effects of auxin during floral development are still unclear. We have shown here that, through analyzing GUS activities of Arabidopsis lines harboring auxin response elements DR5-GUS, auxin was mainly accumulated in the anther during flower stages 10-12. Further studies employing the indoleacetic acid-lysine synthetase (iaaL) coding gene from Pseudomonas syringae subsp. savastanoi under control of the promoter region of Arabidopsis phosphatidylinositol monophosphate 5-kinase 1 gene, which conducts the anther filament-specific expression, showed that block of auxin flow of filaments resulted in shortened filaments and significantly defective pollen grains. Similar phenotype was observed in tobacco plants transformed with the same construct, confirming the effects of auxin flow in filaments on anther development. Detailed studies further revealed that the meiosis process of pollen grain was normal while the mitosis at later stage was significantly defected, indicating the effects of auxin flow in filaments on pollen grain mitosis process. Analysis employing [14C]IAA, as well as the observation on the expression of AtPIN1, coding for auxin efflux carrier, demonstrated the presence of polar auxin transport in anther filaments and pollen grains. Springer, 2006 filaments nationallicence anther nationallicence pollen grain nationallicence auxin nationallicence mitosis nationallicence Feng Xiao-Li National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology, Shanghai Institutes for Biological Sciences/SIBS, Chinese Academy of Sciences, Shanghai, P.R. China aut Ni Wei-Min National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology, Shanghai Institutes for Biological Sciences/SIBS, Chinese Academy of Sciences, Shanghai, P.R. China aut Elge Stephan Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 25, Haus 20, D-14476, Golm, Germany aut Mueller-Roeber Bernd Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 25, Haus 20, D-14476, Golm, Germany aut Xu Zhi-Hong National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology, Shanghai Institutes for Biological Sciences/SIBS, Chinese Academy of Sciences, Shanghai, P.R. China aut Xue Hong-Wei National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology, Shanghai Institutes for Biological Sciences/SIBS, Chinese Academy of Sciences, Shanghai, P.R. China aut Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 215-226 0167-4412 61:1-2<215 2006 61 11103 https://doi.org/10.1007/s11103-006-0005-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0005-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Feng Xiao-Li National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology, Shanghai Institutes for Biological Sciences/SIBS, Chinese Academy of Sciences, Shanghai, P.R. China aut NATIONALLICENCE 700 1- Ni Wei-Min National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology, Shanghai Institutes for Biological Sciences/SIBS, Chinese Academy of Sciences, Shanghai, P.R. China aut NATIONALLICENCE 700 1- Elge Stephan Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 25, Haus 20, D-14476, Golm, Germany aut NATIONALLICENCE 700 1- Mueller-Roeber Bernd Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 25, Haus 20, D-14476, Golm, Germany aut NATIONALLICENCE 700 1- Xu Zhi-Hong National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology, Shanghai Institutes for Biological Sciences/SIBS, Chinese Academy of Sciences, Shanghai, P.R. China aut NATIONALLICENCE 700 1- Xue Hong-Wei National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology, Shanghai Institutes for Biological Sciences/SIBS, Chinese Academy of Sciences, Shanghai, P.R. China aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 215-226 0167-4412 61:1-2<215 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer