caa a22 4500 467894590 CHVBK 20180406152759.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s11103-006-6263-y doi (NATIONALLICENCE)springer-10.1007/s11103-006-6263-y Overexpression of Farnesyl Diphosphate Synthase in Arabidopsis Mitochondria Triggers Light-dependent Lesion Formation and Alters Cytokinin Homeostasis [Elektronische Daten] [David Manzano, Antoni Busquets, Marta Closa, Klára Hoyerová, Hubert Schaller, Miroslav Kamínek, Montserrat Arró, Albert Ferrer] To investigate the role of mitochondrial farnesyl diphosphate synthase (FPS) in plant isoprenoid biosynthesis we characterized transgenic Arabidopsis thaliana plants overexpressing FPS1L isoform. This overexpressed protein was properly targeted to mitochondria yielding a mature and active form of the enzyme of 40kDa. Leaves from transgenic plants grown under continuous light exhibited symptoms of chlorosis and cell death correlating to H2O2 accumulation, and leaves detached from the same plants displayed accelerated senescence. Overexpression of FPS in mitochondria also led to altered leaf cytokinin profile, with a reduction in the contents of physiologically active trans-zeatin- and isopentenyladenine-type cytokinins and their corresponding riboside monophosphates as well as enhanced levels of cis-zeatin 7-glucoside and storage cytokinin O-glucosides. Overexpression of 3-hydroxy-3-methylglutaryl coenzyme A reductase did not prevent chlorosis in plants overexpressing FPS1L, but did rescue accelerated senescence of detached leaves and restored wild-type levels of cytokinins. We propose that the overexpression of FPS1L leads to an enhanced uptake and metabolism of mevalonic acid-derived isopentenyl diphosphate and/or dimethylallyl diphosphate by mitochondria, thereby altering cytokinin homeostasis and causing a mitochondrial dysfunction that renders plants more sensitive to the oxidative stress induced by continuous light. Springer, 2006 Arabidopsis thaliana nationallicence cytokinin nationallicence farnesyl diphosphate synthase nationallicence isoprenoid nationallicence mevalonic acid nationallicence mitochondria nationallicence AOX : alternative oxidase nationallicence CaMV : cauliflower mosaic virus nationallicence Cox : cytochrome oxidase nationallicence DAB : 3,3′-diaminobenzidine nationallicence DMAPP : dimethylallyl diphosphate nationallicence ER : endoplasmic reticulum nationallicence FPP : farnesyl diphosphate nationallicence FPS : farnesyl diphosphate synthase nationallicence GFP : green fluorescent protein nationallicence HMGR : 3-hydroxy-3-methylglutaryl coenzyme A reductase nationallicence IP : isopentenyladenine nationallicence iP7G : N6-(Δ2-isopentenyl)adenine 7-glucoside nationallicence iPR : N6-(Δ2-isopentenyl)adenosine nationallicence iPRMP : N6-(Δ2-isopentenyl)adenosine 5′-monophosphate nationallicence IPP : isopentenyl diphosphate nationallicence IPT : isopentenyltransferase nationallicence MEP : methylerythritol phosphate nationallicence MVA : mevalonic acid nationallicence ROS : reactive oxygen species nationallicence c -Z : cis-zeatin nationallicence t -Z : trans-zeatin nationallicence c -Z7G : cis-zeatin 7-glucoside nationallicence t -Z7G : trans-zeatin 7-glucoside nationallicence t -Z9G : trans-zeatin 9-glucoside nationallicence t -ZOG : trans-zeatin O-glucoside nationallicence c -ZR : cis-zeatin riboside nationallicence t -ZR : trans-zeatin riboside nationallicence t -ZRMP : trans-zeatin riboside 5′-monophosphate nationallicence t -ZROG : trans-zeatin 9-riboside O-glucoside nationallicence Manzano David Departament de Bioquímica i Biologia Molecular, Facultat de Farmàcia, Universitat de Barcelona, Avda. Diagonal 643, 08028, Barcelona, Spain aut Busquets Antoni Departament de Bioquímica i Biologia Molecular, Facultat de Farmàcia, Universitat de Barcelona, Avda. Diagonal 643, 08028, Barcelona, Spain aut Closa Marta Departament de Bioquímica i Biologia Molecular, Facultat de Farmàcia, Universitat de Barcelona, Avda. Diagonal 643, 08028, Barcelona, Spain aut Hoyerová Klára Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojova 135, CZ-165 02, Prague 6, Czech Republic aut Schaller Hubert Département Isoprénoïdes, Institut de Biologie Moléculaire des Plantes (IBMP/CNRS), Institut de Botanique, 28, rue Goethe, 67083, Strasbourg, France aut Kamínek Miroslav Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojova 135, CZ-165 02, Prague 6, Czech Republic aut Arró Montserrat Departament de Bioquímica i Biologia Molecular, Facultat de Farmàcia, Universitat de Barcelona, Avda. Diagonal 643, 08028, Barcelona, Spain aut Ferrer Albert Departament de Bioquímica i Biologia Molecular, Facultat de Farmàcia, Universitat de Barcelona, Avda. Diagonal 643, 08028, Barcelona, Spain aut Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 195-213 0167-4412 61:1-2<195 2006 61 11103 https://doi.org/10.1007/s11103-006-6263-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-6263-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Manzano David Departament de Bioquímica i Biologia Molecular, Facultat de Farmàcia, Universitat de Barcelona, Avda. Diagonal 643, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Busquets Antoni Departament de Bioquímica i Biologia Molecular, Facultat de Farmàcia, Universitat de Barcelona, Avda. Diagonal 643, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Closa Marta Departament de Bioquímica i Biologia Molecular, Facultat de Farmàcia, Universitat de Barcelona, Avda. Diagonal 643, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Hoyerová Klára Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojova 135, CZ-165 02, Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Schaller Hubert Département Isoprénoïdes, Institut de Biologie Moléculaire des Plantes (IBMP/CNRS), Institut de Botanique, 28, rue Goethe, 67083, Strasbourg, France aut NATIONALLICENCE 700 1- Kamínek Miroslav Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojova 135, CZ-165 02, Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Arró Montserrat Departament de Bioquímica i Biologia Molecular, Facultat de Farmàcia, Universitat de Barcelona, Avda. Diagonal 643, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Ferrer Albert Departament de Bioquímica i Biologia Molecular, Facultat de Farmàcia, Universitat de Barcelona, Avda. Diagonal 643, 08028, Barcelona, Spain aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 195-213 0167-4412 61:1-2<195 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer