caa a22 4500 467894604 CHVBK 20180406152759.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s11103-005-5817-8 doi (NATIONALLICENCE)springer-10.1007/s11103-005-5817-8 Direct Interaction between the Tobacco Mosaic Virus Helicase Domain and the ATP-bound Resistance Protein, N Factor during the Hypersensitive Response in Tobacco Plants [Elektronische Daten] [Hirokazu Ueda, Yube Yamaguchi, Hiroshi Sano] Plants cope with pathogens with distinct mechanisms. One example is a gene-for-gene system, in which plants recognize the pathogen molecule by specified protein(s), this being called the R factor. However, mechanisms of interaction between proteins from the host and the pathogen are not completely understood. Here, we analyzed the mode of interaction between the N factor, a tobacco R factor, and the helicase domain (p50) of tobacco mosaic virus (TMV). To this end, domain dissected proteins were prepared and subjected to Agroinfiltration into intact leaves, followed by yeast two hybrid and pull-down assays. The results pointed to three novel features. First, the N factor was found to directly bind to the p50 of TMV, second, ATP was pre-requisite for this interaction, with formation of an ATP/N factor complex, and third, the N factor was shown to possess ATPase activity, which is enhanced by the p50. Moreover, we found that intra- and/or inter-molecular interactions take place in the N factor molecule. This interaction required ATP, and was disrupted by the p50. Based on these results, we propose a following model for the TMV recognition mechanism in tobacco plants. The N factor forms a complex with ATP, to which the helicase domain interacts, and enhances ATP hydrolysis. The resulting ADP/N factor complex then changes its conformation, thereby facilitating further interaction with the down-stream signaling factor(s). This model is consistent with the idea of ‘protein machine'. Springer, 2006 ATP nationallicence helicase nationallicence hypersensitive response nationallicence N factor nationallicence Nicotiana tabacum nationallicence tobacco mosaic virus nationallicence Ueda Hirokazu Research and Education Center for Genetic Information, Nara Institute of Science and Technology, 630-0192, Nara, Japan aut Yamaguchi Yube Research and Education Center for Genetic Information, Nara Institute of Science and Technology, 630-0192, Nara, Japan aut Sano Hiroshi Research and Education Center for Genetic Information, Nara Institute of Science and Technology, 630-0192, Nara, Japan aut Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 31-45 0167-4412 61:1-2<31 2006 61 11103 https://doi.org/10.1007/s11103-005-5817-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-5817-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ueda Hirokazu Research and Education Center for Genetic Information, Nara Institute of Science and Technology, 630-0192, Nara, Japan aut NATIONALLICENCE 700 1- Yamaguchi Yube Research and Education Center for Genetic Information, Nara Institute of Science and Technology, 630-0192, Nara, Japan aut NATIONALLICENCE 700 1- Sano Hiroshi Research and Education Center for Genetic Information, Nara Institute of Science and Technology, 630-0192, Nara, Japan aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 31-45 0167-4412 61:1-2<31 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer