caa a22 4500 467894612 CHVBK 20180406152759.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s11103-005-0001-8 doi (NATIONALLICENCE)springer-10.1007/s11103-005-0001-8 AtRLI2 is an Endogenous Suppressor of RNA Silencing [Elektronische Daten] [Cecilia Sarmiento, Lenne Nigul, Jekaterina Kazantseva, Marju Buschmann, Erkki Truve] RNA silencing is a mechanism involved in gene regulation during development and anti-viral defense in plants and animals. Although many viral suppressors of this mechanism have been described up to now, this is not the case for endogenous suppressors. We have identified a novel endogenous suppressor in plants: RNase L inhibitor (RLI) of Arabidopsis thaliana. RLI is a very conserved protein among eukaryotes and archaea. It was first known as component of the interferon-induced mammalian 2′-5′ oligoadenylate (2-5A) anti-viral pathway. This protein is in several organisms responsible for essential functions, which are not related to the 2-5A pathway, like ribosome biogenesis and translation initiation. Arabidopsis has two RLI paralogs. We have described in detail the expression pattern of one of these paralogs (AtRLI2), which is ubiquitously expressed in all plant organs during different developmental stages. Infiltrating Nicotiana benthamiana green fluorescent protein (GFP)-transgenic line with Agrobacterium strains harboring GFP and AtRLI2, we proved that AtRLI2 suppresses silencing at the local and at the systemic level, reducing drastically the amount of GFP small interfering RNAs. Springer, 2006 agroinfiltration nationallicence Arabidopsis thaliana nationallicence expression analysis nationallicence RNA interference nationallicence RNase L inhibitor nationallicence siRNA nationallicence 2-5A : 2′-5′ oligoadenylate nationallicence Ago : Argonaute protein nationallicence AtRLI2 : Arabidopsis thaliana gene At4g19210 nationallicence dpi : days post-infiltration nationallicence dsRNA : double-stranded RNA nationallicence GFP : green fluorescent protein nationallicence nt : nucleotide nationallicence RISC : RNA-induced silencing complex nationallicence RdRP : RNA-dependent RNA polymerase nationallicence RLI : RNase L inhibitor nationallicence RNAi : RNA interference nationallicence RPA : RNase protection assay nationallicence siRNA : small interfering RNA nationallicence UTR : untranslated region nationallicence Sarmiento Cecilia Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 19086, Tallinn, Estonia aut Nigul Lenne Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 19086, Tallinn, Estonia aut Kazantseva Jekaterina Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 19086, Tallinn, Estonia aut Buschmann Marju Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 19086, Tallinn, Estonia aut Truve Erkki Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 19086, Tallinn, Estonia aut Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 153-163 0167-4412 61:1-2<153 2006 61 11103 https://doi.org/10.1007/s11103-005-0001-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-0001-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sarmiento Cecilia Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 19086, Tallinn, Estonia aut NATIONALLICENCE 700 1- Nigul Lenne Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 19086, Tallinn, Estonia aut NATIONALLICENCE 700 1- Kazantseva Jekaterina Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 19086, Tallinn, Estonia aut NATIONALLICENCE 700 1- Buschmann Marju Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 19086, Tallinn, Estonia aut NATIONALLICENCE 700 1- Truve Erkki Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 19086, Tallinn, Estonia aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 153-163 0167-4412 61:1-2<153 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer