caa a22 4500 467894620 CHVBK 20180406152759.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s11103-005-6102-6 doi (NATIONALLICENCE)springer-10.1007/s11103-005-6102-6 CASAR82A, a Pathogen-induced Pepper SAR8.2, Exhibits an Antifungal Activity and its Overexpression Enhances Disease Resistance and Stress Tolerance [Elektronische Daten] [Sung Lee, Byung Hwang] Pepper SAR8.2 gene (CASAR82A) was previously reported to be locally or systemically induced in pepper plants by biotic and abiotic stresses. In this study, the physiological and molecular functions of the pepper SAR8.2 protein in the plant defense responses were investigated by generating Arabidopsis transgenic lines overexpressing the CASAR82A gene. The transgenic Arabidopsis plants grew faster than the wild-type plants, indicating that the CASAR82A gene was involved in plant development. The ectopic expression of CASAR82A in Arabidopsis was accompanied by the expression of the Arabidopsis pathogenesis-related (PR)-genes including AtPR-1, AtPR-4 and AtPR-5. CASAR82A overexpression enhanced the resistance against infections by Pseudomonas syringae pv. tomato, Fusarium oxysporum f.sp. matthiolae or Botrytis cinerea. The transgenic plants also exhibited increased NaCl and drought tolerance during all growth stages. Moreover, the methyl viologen test showed that the transgenic plants were tolerant to oxidative stress. The purified recombinant CASAR82A protein and crude protein extracts of the transgenic plants exhibited antifungal activity against some phytopathogenic fungi, indicating that the enhanced resistance of the transgenic plants to fungal pathogen infection may be due to the antifungal effect of SAR8.2 protein. Springer, 2006 antifungal activity nationallicence disease resistance nationallicence pepper nationallicence SAR8.2 nationallicence stress tolerance nationallicence Lee Sung Laboratory of Molecular Plant Pathology, College of Life and Environmental Sciences, Korea University, 136-713, Sungbuk-ku, Anam-dong, Seoul, Republic of Korea aut Hwang Byung Laboratory of Molecular Plant Pathology, College of Life and Environmental Sciences, Korea University, 136-713, Sungbuk-ku, Anam-dong, Seoul, Republic of Korea aut Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 95-109 0167-4412 61:1-2<95 2006 61 11103 https://doi.org/10.1007/s11103-005-6102-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-6102-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Sung Laboratory of Molecular Plant Pathology, College of Life and Environmental Sciences, Korea University, 136-713, Sungbuk-ku, Anam-dong, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Hwang Byung Laboratory of Molecular Plant Pathology, College of Life and Environmental Sciences, Korea University, 136-713, Sungbuk-ku, Anam-dong, Seoul, Republic of Korea aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 95-109 0167-4412 61:1-2<95 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer