caa a22 4500 467894647 CHVBK 20180406152759.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s11103-005-6173-4 doi (NATIONALLICENCE)springer-10.1007/s11103-005-6173-4 A Microarray-based Detection System for Genetically Modified (GM) Food Ingredients [Elektronische Daten] [Serge Leimanis, Marta Hernández, Sophie Fernández, Francine Boyer, Malcolm Burns, Shirin Bruderer, Thomas Glouden, Neil Harris, Othmar Kaeppeli, Patrick Philipp, Maria Pla, Pere Puigdomènech, Marc Vaitilingom, Yves Bertheau, José Remacle] A multiplex DNA microarray chip was developed for simultaneous identification of nine genetically modified organisms (GMOs), five plant species and three GMO screening elements, i.e. the 35S promoter, the nos terminator and the nptII gene. The chips also include several controls, such as that for the possible presence of CaMV. The on-chip detection was performed directly with PCR amplified products. Particular emphasis was placed on the reduction of the number of PCR reactions required and on the number of primers present per amplification tube. The targets were biotin labelled and the arrays were detected using a colorimetric methodology. Specificity was provided by specific capture probes designed for each GMO and for the common screening elements. The sensitivity of the assay was tested by experiments carried out in five different laboratories. The limit of detection was lower than 0.3% GMO for all tests and in general around 0.1% for most GMOs. The chip detection system complies with the requirements of current EU regulations and other countries where thresholds are established for the labelling of GMO. Springer, 2006 colorimetric nationallicence detection nationallicence GMO nationallicence GMOchips nationallicence microarray nationallicence CaMV : cauliflower mosaic virus nationallicence CTAB : hexadecyltrimethylammonium bromide nationallicence DNA : deoxyribonucleic acid nationallicence EC : European Commission nationallicence EU : European Union nationallicence GMO : genetically modified organism nationallicence NptII : neomycin phospho transferase originating from Streptomyces hygroscopicus nationallicence P-35S : promoter 35S originating from cauliflower mosaic virus nationallicence Pat : phosphinotricin-N-acetyl transferase nationallicence PCR : polymerase chain reaction nationallicence RRS : roundup ready® soybean nationallicence T35S : terminator 35S originating from cauliflower mosaic virus nationallicence Ta : annealing temperature nationallicence T-nos : terminator nopaline synthase originating from Agrobacterium tumefaciens nationallicence UNG : uracil-N-glycosylase nationallicence Leimanis Serge Unité de Recherche en Biologie Cellulaire (URBC), Faculté Universitaire Notre Dame de la Paix, 61, rue de Bruxelles, 5000, Namur, Belgium aut Hernández Marta Molecular Genetics Department, Instituto de Biología Molecular de Barcelona (IBMB) Consejo Superior de Investigaciones Científicas (CSIC), Jordi Girona Salgado 18-26, 08034, Barcelona, Spain aut Fernández Sophie Laboratoire de méthodologies de la détection des OGM, INRA Versailles, Route de Saint Cyr RD 10, 78026, Versailles Cedex, France aut Boyer Francine Laboratoire de méthodologies de la détection des OGM, INRA Versailles, Route de Saint Cyr RD 10, 78026, Versailles Cedex, France aut Burns Malcolm LGC Limited, Queens Road, TW11 OLY, Teddington, Middlesex, UK aut Bruderer Shirin Agency BATS, Clarastrasse 13, 4058, Base, Switzerland aut Glouden Thomas Unité de Recherche en Biologie Cellulaire (URBC), Faculté Universitaire Notre Dame de la Paix, 61, rue de Bruxelles, 5000, Namur, Belgium aut Harris Neil LGC Limited, Queens Road, TW11 OLY, Teddington, Middlesex, UK aut Kaeppeli Othmar Agency BATS, Clarastrasse 13, 4058, Base, Switzerland aut Philipp Patrick Laboratoire Interrégional, DGCCRF, 67 Chemin du routoir, 67400, Illkirch-Graffenstaden, France aut Pla Maria Molecular Genetics Department, Instituto de Biología Molecular de Barcelona (IBMB) Consejo Superior de Investigaciones Científicas (CSIC), Jordi Girona Salgado 18-26, 08034, Barcelona, Spain aut Puigdomènech Pere Molecular Genetics Department, Instituto de Biología Molecular de Barcelona (IBMB) Consejo Superior de Investigaciones Científicas (CSIC), Jordi Girona Salgado 18-26, 08034, Barcelona, Spain aut Vaitilingom Marc TEPRAL—Brasseries KRONENBOURG, 68, route d'Oberhausbergen, 67037, Strasbourg, France aut Bertheau Yves Laboratoire de méthodologies de la détection des OGM, INRA Versailles, Route de Saint Cyr RD 10, 78026, Versailles Cedex, France aut Remacle José Unité de Recherche en Biologie Cellulaire (URBC), Faculté Universitaire Notre Dame de la Paix, 61, rue de Bruxelles, 5000, Namur, Belgium aut Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 123-139 0167-4412 61:1-2<123 2006 61 11103 https://doi.org/10.1007/s11103-005-6173-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-6173-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Leimanis Serge Unité de Recherche en Biologie Cellulaire (URBC), Faculté Universitaire Notre Dame de la Paix, 61, rue de Bruxelles, 5000, Namur, Belgium aut NATIONALLICENCE 700 1- Hernández Marta Molecular Genetics Department, Instituto de Biología Molecular de Barcelona (IBMB) Consejo Superior de Investigaciones Científicas (CSIC), Jordi Girona Salgado 18-26, 08034, Barcelona, Spain aut NATIONALLICENCE 700 1- Fernández Sophie Laboratoire de méthodologies de la détection des OGM, INRA Versailles, Route de Saint Cyr RD 10, 78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Boyer Francine Laboratoire de méthodologies de la détection des OGM, INRA Versailles, Route de Saint Cyr RD 10, 78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Burns Malcolm LGC Limited, Queens Road, TW11 OLY, Teddington, Middlesex, UK aut NATIONALLICENCE 700 1- Bruderer Shirin Agency BATS, Clarastrasse 13, 4058, Base, Switzerland aut NATIONALLICENCE 700 1- Glouden Thomas Unité de Recherche en Biologie Cellulaire (URBC), Faculté Universitaire Notre Dame de la Paix, 61, rue de Bruxelles, 5000, Namur, Belgium aut NATIONALLICENCE 700 1- Harris Neil LGC Limited, Queens Road, TW11 OLY, Teddington, Middlesex, UK aut NATIONALLICENCE 700 1- Kaeppeli Othmar Agency BATS, Clarastrasse 13, 4058, Base, Switzerland aut NATIONALLICENCE 700 1- Philipp Patrick Laboratoire Interrégional, DGCCRF, 67 Chemin du routoir, 67400, Illkirch-Graffenstaden, France aut NATIONALLICENCE 700 1- Pla Maria Molecular Genetics Department, Instituto de Biología Molecular de Barcelona (IBMB) Consejo Superior de Investigaciones Científicas (CSIC), Jordi Girona Salgado 18-26, 08034, Barcelona, Spain aut NATIONALLICENCE 700 1- Puigdomènech Pere Molecular Genetics Department, Instituto de Biología Molecular de Barcelona (IBMB) Consejo Superior de Investigaciones Científicas (CSIC), Jordi Girona Salgado 18-26, 08034, Barcelona, Spain aut NATIONALLICENCE 700 1- Vaitilingom Marc TEPRAL—Brasseries KRONENBOURG, 68, route d'Oberhausbergen, 67037, Strasbourg, France aut NATIONALLICENCE 700 1- Bertheau Yves Laboratoire de méthodologies de la détection des OGM, INRA Versailles, Route de Saint Cyr RD 10, 78026, Versailles Cedex, France aut NATIONALLICENCE 700 1- Remacle José Unité de Recherche en Biologie Cellulaire (URBC), Faculté Universitaire Notre Dame de la Paix, 61, rue de Bruxelles, 5000, Namur, Belgium aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 123-139 0167-4412 61:1-2<123 2006 61 11103 Metadata rights reserved 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