caa a22 4500 467894663 CHVBK 20180406152759.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s11103-006-6268-6 doi (NATIONALLICENCE)springer-10.1007/s11103-006-6268-6 Uncovering the Post-embryonic Role of Embryo Essential Genes in Arabidopsis using the Controlled Induction of Visibly Marked Genetic Mosaics: EMB506 , an Illustration [Elektronische Daten] [Satu Latvala-Kilby, Nigel Kilby] The embryo essential gene EMB506 plays a crucial role in the transition of the Arabidopsis embryo from radial symmetry to bilateral symmetry just prior to the early heart stage of development. In addition to influencing embryo development EMB506 also affects chloroplast biogenesis. To further investigate the role of EMB506 gene expression in Arabidopsis we have generated green fluorescent protein (GFP) marked emb506 mosaic sectors at temporally defined stages during embryogenesis and additionally during various stages of vegetative growth, in otherwise phenotypically wild-type plants. We confirm the essential requirement for EMB506 gene expression in chloroplast biogenesis as reflected by the decreased chlorophyll content in emb506 mosaic sectors. We also show that the influence of EMB506 gene expression as it impinges on chloroplast biogenesis is first relevant at an intermediate stage in embryogenesis and that the role of EMB506 gene expression in chloroplast biogenesis is distinct from the essential role of EMB506 gene expression during early embryo development. By inducing emb506 mosaicism after the essential requirement for EMB506 gene expression in embryogenesis and also during vegetative growth we reveal that EMB506 gene expression additionally is required for correct cotyledon-, true leaf- and cauline leaf margin development. The strategy that we describe can be tailored to the mosaic analysis of any cloned EMB gene for which a corresponding mutant exists and can be applied to the mosaic analysis of mutant lethal genes in general. Springer, 2006 EMB506 nationallicence embryo essential nationallicence genetic mosaic nationallicence lateral organ margin nationallicence post-embryonic role nationallicence BL : T-DNA left border nationallicence bp : base pair nationallicence BR : T-DNA right border nationallicence cDNA : complementary DNA nationallicence EMB : embryo essential nationallicence 506 cDNA : EMB506 cDNA nationallicence DAP : day after pollination nationallicence DAS : day after stratification nationallicence FLP : FLP recombinase nationallicence FRT : FLP recognition target nationallicence GFP : green fluorescent protein nationallicence Hyg : hygromycin phosphotransferase coding region nationallicence kb : kilo base pair nationallicence MWT : molecular weight nationallicence nos : nopaline synthase polyadenylation transcription termination sequence nationallicence ocs : octopine synthase polyadenylation transcription termination sequence nationallicence P ABI3 : ABI3 promoter nationallicence P 35S : 35S cauliflower mosaic virus promoter nationallicence P 506 : EMB506 promoter nationallicence PCR : polymerase chain reaction nationallicence RT-PCR : reverse transcription PCR nationallicence SAM : shoot apical meristem nationallicence T-DNA : transfer DNA nationallicence Latvala-Kilby Satu MTT Agrifood Research, Plant Production, 31600, Jokioinen, Finland aut Kilby Nigel Boreal Plant Breeding Ltd., 31600, Jokioinen, Finland aut Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 179-194 0167-4412 61:1-2<179 2006 61 11103 https://doi.org/10.1007/s11103-006-6268-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-6268-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Latvala-Kilby Satu MTT Agrifood Research, Plant Production, 31600, Jokioinen, Finland aut NATIONALLICENCE 700 1- Kilby Nigel Boreal Plant Breeding Ltd., 31600, Jokioinen, Finland aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 179-194 0167-4412 61:1-2<179 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer