caa a22 4500 467894671 CHVBK 20180406152759.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s11103-005-6002-9 doi (NATIONALLICENCE)springer-10.1007/s11103-005-6002-9 A LINE-type Retrotransposon Active in Meristem Stem Cells Causes Heritable Transpositions in the Sweet Potato Genome [Elektronische Daten] [Hiroki Yamashita, Makoto Tahara] We isolated a LINE-type retrotransposon, LIb, which showed high transposition activity in sweet potato callus. A copy transposed in the callus was 6303bp in length and showed key features of a LINE element. Apparently full-length copies sharing the 5′ UTR sequence with the 6303-bp copy increased dramatically in the callus as several original copies in the sweet potato genome. These apparently full-length copies had almost identical sequences to other transposed copies, many of which were truncated at the 5′ end upon transposition. These results indicate that active LIb is confined to a single LINE family, and that members containing a long functional 5′ UTR are present in limited numbers in the sweet potato genome. This is despite their copy numbers being estimated at over 100. The transcription of LIb was not completely suppressed, even in wild-type plants. Spontaneous transpositions were found among local variant lines of the cultivar Koukei14, from which the callus with high LIb activity was derived. Meristem culture of this cultivar appeared to facilitate transpositions of LIb in a mericlone plant. This is the first experimental demonstration of retrotransposition in a plant species without the imposition of cell differentiation. LIb transpositions appear to occur in single founder cells in the meristem because the LIb insertion was found throughout mericlone plant tissues. Transpositional activities in meristem cells might be essential characteristics of plant retrotransposons that cause heritable changes in host plant genomes and genetic systems. Springer, 2006 LINE nationallicence meristem nationallicence retrotransposon nationallicence stem cell nationallicence sweet potato nationallicence transposition nationallicence LINE : long interspersed nucleotide element nationallicence LTR : long terminal repeat nationallicence MCS : multiple cloning site nationallicence ORF : open reading frame nationallicence RACE : rapid amplification of cDNA ends nationallicence RTase : reverse transcriptase nationallicence RT-PCR : reverse transcriptase-polymerase chain reaction nationallicence SINE : short interspersed nucleotide element nationallicence S-SAP : sequence-specific amplification polymorphism nationallicence TPRT : target-primed reverse transcription nationallicence TSD : target site duplication nationallicence UTR : untranslated region nationallicence Yamashita Hiroki Graduate School of Natural Science and Technology, Okayama University, 1-1-1 Tsushimanaka, Okayama, Japan aut Tahara Makoto Graduate School of Natural Science and Technology, Okayama University, 1-1-1 Tsushimanaka, Okayama, Japan aut Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 79-84 0167-4412 61:1-2<79 2006 61 11103 https://doi.org/10.1007/s11103-005-6002-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-6002-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamashita Hiroki Graduate School of Natural Science and Technology, Okayama University, 1-1-1 Tsushimanaka, Okayama, Japan aut NATIONALLICENCE 700 1- Tahara Makoto Graduate School of Natural Science and Technology, Okayama University, 1-1-1 Tsushimanaka, Okayama, Japan aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 79-84 0167-4412 61:1-2<79 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer