caa a22 4500 46789468X CHVBK 20180406152759.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s11103-006-0028-5 doi (NATIONALLICENCE)springer-10.1007/s11103-006-0028-5 Identification and Characterization of a Host Reversibly Glycosylated Peptide that Interacts with the Tomato leaf curl virus V1 Protein [Elektronische Daten] [Luke Selth, Satish Dogra, M. Rasheed, John. Randles, M. Rezaian] Monopartite geminiviruses of the genus Begomovirus have two virion-sense genes, V1 and V2. V2 encodes the viral coat protein, but the function of V1 is largely unknown, although some studies suggest that it may play a role in cell-to-cell movement. Yeast two-hybrid technology was used to identify possible host binding partners of V1 from Tomato leaf curl virus (TLCV) to better understand its function. A protein closely related to a family of plant reversibly glycosylated peptides, designated SlUPTG1, was found to interact with V1 in yeast and in vitro. SlUPTG1 may function endogenously in the synthesis of cell wall polysaccharides, since a bacterially expressed form of the protein acted as an autocatalytic glycosyltransferase in␣vitro, a SlUPTG1:GFP fusion protein localized to the cell wall, and expression of SlUPTG1 appeared to be highest in actively dividing tissues. However, expression of SlUPTG1 in a transient TLCV replication assay increased the accumulation of viral DNA, suggesting that this host factor also plays a role in viral infection. Together, these data provide new insight into the role of V1 in TLCV infection and reveal another host pathway which geminiviruses may manipulate to achieve an efficient infection. Springer, 2006 cell wall biosynthesis nationallicence geminivirus nationallicence reversibly glycosylated peptide nationallicence virus movement nationallicence virus replication nationallicence ACMV : African cassava mosaic virus nationallicence AD : activation domain nationallicence CaMV : Cauliflower mosaic virus nationallicence CAT : chloramphenicol acetlytransferase nationallicence CBP : calmodulin-binding peptide nationallicence ER : endoplasmic reticulum nationallicence GFP : green fluorescent protein nationallicence GUS : ß-glucuronidase nationallicence Ni-NTA : nickel-nitriloacetic acid nationallicence ORF : open-reading frame nationallicence Pd : plasmodesmata nationallicence PM : plasma membrane nationallicence RGP : reversibly glycosylated peptide nationallicence TLCV : Tomato leaf curl virus nationallicence TYLCSV : Tomato yellow leaf curl Sardinia virus nationallicence UPTG : UDP-glucose:protein transglucosylase nationallicence Selth Luke Horticulture Unit, CSIRO Plant Industry, P.O. Box 350, 5064, Glen Osmond, SA, Australia aut Dogra Satish Horticulture Unit, CSIRO Plant Industry, P.O. Box 350, 5064, Glen Osmond, SA, Australia aut Rasheed M. Horticulture Unit, CSIRO Plant Industry, P.O. Box 350, 5064, Glen Osmond, SA, Australia aut Randles John School of Agriculture and Wine, The University of Adelaide, 5005, Adelaide, SA, Australia aut Rezaian M. Horticulture Unit, CSIRO Plant Industry, P.O. Box 350, 5064, Glen Osmond, SA, Australia aut Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 297-310 0167-4412 61:1-2<297 2006 61 11103 https://doi.org/10.1007/s11103-006-0028-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-0028-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Selth Luke Horticulture Unit, CSIRO Plant Industry, P.O. Box 350, 5064, Glen Osmond, SA, Australia aut NATIONALLICENCE 700 1- Dogra Satish Horticulture Unit, CSIRO Plant Industry, P.O. Box 350, 5064, Glen Osmond, SA, Australia aut NATIONALLICENCE 700 1- Rasheed M. Horticulture Unit, CSIRO Plant Industry, P.O. Box 350, 5064, Glen Osmond, SA, Australia aut NATIONALLICENCE 700 1- Randles John School of Agriculture and Wine, The University of Adelaide, 5005, Adelaide, SA, Australia aut NATIONALLICENCE 700 1- Rezaian M. Horticulture Unit, CSIRO Plant Industry, P.O. Box 350, 5064, Glen Osmond, SA, Australia aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 297-310 0167-4412 61:1-2<297 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer