caa a22 4500 46789471X CHVBK 20180406152759.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s11103-005-5773-3 doi (NATIONALLICENCE)springer-10.1007/s11103-005-5773-3 Copy Number of Bean Mitochondrial Genes Estimated by Real-time PCR does not Correlate with the Number of Gene Loci and Transcript Levels [Elektronische Daten] [Magdalena Woloszynska, Beata Kmiec, Pawel Mackiewicz, Hanna Janska] Structural rearrangements characteristic for plant mitochondrial DNA often result in the appearance of genes in new genomic environments. The determination of the real number of gene copies is difficult since the in vivo structure of plant mitochondrial genomes is questionable. It is still uncertain whether the gene copy number regulates transcription in plant mitochondria. Using the real-time PCR technique we have quantified the copies of mitochondrial genes and their transcripts in four related Phaseolus vulgaris lines. We found low intergenomic variation both in the copy number of particular genes and the abundance of their transcripts, while the intragenomic differences between copy numbers and transcripts levels of various genes were much higher. Furthermore, we found that the appearance of a gene in a new location is not correlated with a proportional increase in its copy number estimated by real-time PCR. This observation seems to result from gene dosage compensation which is probably associated with the multimolecular plant mitochondrial genome structure and particularly with the recombinogenic activity of large repeats. Based on the relative gene copy numbers we propose the existence of two types of Phaseolus mitochondrial genomes: one associated with fertility and the other inducing cytoplasmic male sterility. We also show that there is no correlation between the observed number of copies of the analyzed genes and the steady-state level of their transcripts. Springer, 2006 gene copy number nationallicence Phaseolus vulgaris nationallicence plant mitochondria nationallicence real-time PCR nationallicence steady-state transcript level nationallicence transcription regulation nationallicence atp9 : gene encoding subunit 9 of ATP synthase nationallicence atpA : gene encoding alpha-subunit of ATP synthase nationallicence CMS : cytoplasmic male sterility nationallicence cob : gene encoding cytochrome b nationallicence coxIII : gene encoding subunit 3 of cytochrome oxidase nationallicence mtDNA : mitochondrial DNA nationallicence pvs : Phaseolus vulgaris sterility sequence nationallicence rrn18 : gene encoding 18S rRNA nationallicence r s : The Spearman's rank correlation coefficient nationallicence trnfM : gene encoding initiator methionine tRNA nationallicence Woloszynska Magdalena Department of Cell Molecular Biology, Institute of Biochemistry and Molecular Biology, University of Wroclaw, ul. Przybyszewskiego 63/77, 51-148, Wroclaw, Poland aut Kmiec Beata Department of Cell Molecular Biology, Institute of Biochemistry and Molecular Biology, University of Wroclaw, ul. Przybyszewskiego 63/77, 51-148, Wroclaw, Poland aut Mackiewicz Pawel Department of Genomics, Institute of Genetics and Microbiology, University of Wroclaw, ul. Przybyszewskiego 63/77, 51-148, Wroclaw, Poland aut Janska Hanna Department of Cell Molecular Biology, Institute of Biochemistry and Molecular Biology, University of Wroclaw, ul. Przybyszewskiego 63/77, 51-148, Wroclaw, Poland aut Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 1-12 0167-4412 61:1-2<1 2006 61 11103 https://doi.org/10.1007/s11103-005-5773-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-5773-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Woloszynska Magdalena Department of Cell Molecular Biology, Institute of Biochemistry and Molecular Biology, University of Wroclaw, ul. Przybyszewskiego 63/77, 51-148, Wroclaw, Poland aut NATIONALLICENCE 700 1- Kmiec Beata Department of Cell Molecular Biology, Institute of Biochemistry and Molecular Biology, University of Wroclaw, ul. Przybyszewskiego 63/77, 51-148, Wroclaw, Poland aut NATIONALLICENCE 700 1- Mackiewicz Pawel Department of Genomics, Institute of Genetics and Microbiology, University of Wroclaw, ul. Przybyszewskiego 63/77, 51-148, Wroclaw, Poland aut NATIONALLICENCE 700 1- Janska Hanna Department of Cell Molecular Biology, Institute of Biochemistry and Molecular Biology, University of Wroclaw, ul. Przybyszewskiego 63/77, 51-148, Wroclaw, Poland aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 1-12 0167-4412 61:1-2<1 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer