caa a22 4500 467894728 CHVBK 20180406152759.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s11103-005-5992-7 doi (NATIONALLICENCE)springer-10.1007/s11103-005-5992-7 Characterizations of a Hypomorphic Argonaute1 Mutant Reveal Novel AGO1 Functions in Arabidopsis Lateral Organ Development [Elektronische Daten] [Li Yang, Weihua Huang, Hua Wang, Run Cai, Yuquan Xu, Hai Huang] Genes that encode the ARGONAUTE (AGO) proteins make up a highly conserved family, and several members in the family have been defined to function in posttranscriptional gene silencing (PTGS) in plants, quelling in fungi and RNAi in animals. The Arabidopsis AGO1 gene has been demonstrated to be crucial in multiple RNA silencing pathways (PTGS, microRNA and trans-acting siRNA pathways); however, its biological functions do not seem to be fully addressed. Here we report characterizations of a new hypomorphic ago1 allele, ago1-37, and show novel AGO1 functions important in lateral organ development. We found that double mutants combining ago1-37 with asymmetric leaves1 (as1) or asymmetric leaves2 (as2) produced more severe phenotypes than the single mutants, indicating that AGO1 genetically interacts with AS1 and AS2 for plant development. Similar to the previously characterized mutants rdr6, sgs3 and zippy, which are deficient in the trans-acting siRNA activity, ago1-37 also showed an earlier phase transition from juvenile to adult leaves. Moreover, based on the detailed phenotypic analyses of single and double mutant plants, we found that the AGO1 functions are required for repressing several class I KNOTTED1-like homeobox (KNOX) genes in leaves, and for specifying both adaxial and abaxial identities of the leaf and petal. Our results demonstrate several important AGO1 functions in plant lateral organ development. Springer, 2006 Arabidopsis nationallicence ARGONAUTE1 nationallicence KNOX genes nationallicence leaf polarity nationallicence posttranscriptional gene silencing nationallicence PTGS : posttranscriptional gene silencing nationallicence RdRP : RNA-dependent RNA polymerase nationallicence RISC : RNA induced silencing complex nationallicence Yang Li National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032, Shanghai, China aut Huang Weihua National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032, Shanghai, China aut Wang Hua National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032, Shanghai, China aut Cai Run School of Agriculture and Biology, Shanghai Jiao Tong University, 2678 Qi Xing Road, 201101, Shanghai, China aut Xu Yuquan College of Life Science and Biotechnology, Shanghai Jiao Tong University, 1954 Hua Shan Road, 200030, Shanghai, China aut Huang Hai National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032, Shanghai, China aut Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 63-78 0167-4412 61:1-2<63 2006 61 11103 https://doi.org/10.1007/s11103-005-5992-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-005-5992-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Li National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032, Shanghai, China aut NATIONALLICENCE 700 1- Huang Weihua National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032, Shanghai, China aut NATIONALLICENCE 700 1- Wang Hua National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032, Shanghai, China aut NATIONALLICENCE 700 1- Cai Run School of Agriculture and Biology, Shanghai Jiao Tong University, 2678 Qi Xing Road, 201101, Shanghai, China aut NATIONALLICENCE 700 1- Xu Yuquan College of Life Science and Biotechnology, Shanghai Jiao Tong University, 1954 Hua Shan Road, 200030, Shanghai, China aut NATIONALLICENCE 700 1- Huang Hai National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032, Shanghai, China aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 61/1-2(2006-05-01), 63-78 0167-4412 61:1-2<63 2006 61 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer