caa a22 4500 467894736 CHVBK 20180406152759.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s11103-006-9050-x doi (NATIONALLICENCE)springer-10.1007/s11103-006-9050-x Functional analysis of members of the isoflavone and isoflavanone O -methyltransferase enzyme families from the model legume Medicago truncatula [Elektronische Daten] [Bettina Deavours, Chang-Jun Liu, Marina Naoumkina, Yuhong Tang, Mohamed Farag, Lloyd Sumner, Joseph Noel, Richard Dixon] Previous studies have identified two distinct O-methyltransferases (OMTs) implicated in isoflavonoid biosynthesis in Medicago species, a 7-OMT methylating the A-ring 7-hydroxyl of the isoflavone daidzein and a 4'-OMT methylating the B-ring 4′-hydroxyl of 2,7,4′-trihydroxyisoflavanone. Genes related to these OMTs from the model legume Medicago truncatula cluster as separate branches of the type I plant small molecule OMT family. To better understand the possible functions of these related OMTs in secondary metabolism in M. truncatula, seven of the OMTs were expressed in E. coli, purified, and their in vitro substrate preferences determined. Many of the enzymes display promiscuous activities, and some exhibit dual regio-specificity for the 4′ and 7-hydroxyl moieties of the isoflavonoid nucleus. Protein structure homology modeling was used to help rationalize these catalytic activities. Transcripts encoding the different OMT genes exhibited differential tissue-specific and infection- or elicitor-induced expression, but not always in parallel with changes in expression of confirmed genes of the isoflavonoid pathway. The results are discussed in relation to the potential in vivo functions of these OMTs based on our current understanding of the phytochemistry of M. truncatula, and the difficulties associated with gene annotation in plant secondary metabolism. Springer Science+Business Media B.V., 2006 Isoflavonoid nationallicence O -methyltransferase nationallicence Secondary metabolism nationallicence Molecular modeling nationallicence Gene family nationallicence EST : expressed sequence tag nationallicence IFS : isoflavone synthase nationallicence MeJA : methyl jasmonate nationallicence OMT : O-methyltransferase nationallicence TC : tentative consensus nationallicence YE : yeast elicitor nationallicence Deavours Bettina Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, 73401, Ardmore, OK, USA aut Liu Chang-Jun Howard Hughes Medical Institute, Jack Skirball Chemical Biology and Proteomics Laboratory, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, 92036, La Jolla, CA, USA aut Naoumkina Marina Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, 73401, Ardmore, OK, USA aut Tang Yuhong Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, 73401, Ardmore, OK, USA aut Farag Mohamed Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, 73401, Ardmore, OK, USA aut Sumner Lloyd Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, 73401, Ardmore, OK, USA aut Noel Joseph Howard Hughes Medical Institute, Jack Skirball Chemical Biology and Proteomics Laboratory, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, 92036, La Jolla, CA, USA aut Dixon Richard Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, 73401, Ardmore, OK, USA aut Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 715-733 0167-4412 62:4-5<715 2006 62 11103 https://doi.org/10.1007/s11103-006-9050-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9050-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Deavours Bettina Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, 73401, Ardmore, OK, USA aut NATIONALLICENCE 700 1- Liu Chang-Jun Howard Hughes Medical Institute, Jack Skirball Chemical Biology and Proteomics Laboratory, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, 92036, La Jolla, CA, USA aut NATIONALLICENCE 700 1- Naoumkina Marina Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, 73401, Ardmore, OK, USA aut NATIONALLICENCE 700 1- Tang Yuhong Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, 73401, Ardmore, OK, USA aut NATIONALLICENCE 700 1- Farag Mohamed Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, 73401, Ardmore, OK, USA aut NATIONALLICENCE 700 1- Sumner Lloyd Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, 73401, Ardmore, OK, USA aut NATIONALLICENCE 700 1- Noel Joseph Howard Hughes Medical Institute, Jack Skirball Chemical Biology and Proteomics Laboratory, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, 92036, La Jolla, CA, USA aut NATIONALLICENCE 700 1- Dixon Richard Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, 73401, Ardmore, OK, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 715-733 0167-4412 62:4-5<715 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer