caa a22 4500 467894752 CHVBK 20180406152759.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s11103-006-9042-x doi (NATIONALLICENCE)springer-10.1007/s11103-006-9042-x Linear molecules of tobacco ptDNA end at known replication origins and additional loci [Elektronische Daten] [Lars Scharff, Hans-Ulrich Koop] Higher plant plastid DNA (ptDNA) is generally described as a double-stranded circular molecule of the size of the monomer of the plastid genome. Also, the substrates and products of ptDNA replication are generally assumed to be circular molecules. Linear or partly linear ptDNA molecules were detected in our present study using pulsed-field gel electrophoresis and Southern blotting of ptDNA restricted with ‘single cutter' restriction enzymes. These linear DNA molecules show discrete end points which were mapped using appropriate probes. One possible explanation of discrete ends would be that they represent origins of replication. Indeed, some of the mapped ends correlate well with the known origins of replication of tobacco plastids, i.e. both of the oriA sequences and—less pronouncedly—with the oriB elements. Other ends correspond to replication origins that were described for Oenothera hookeri, Zea mays, Glycine max and Chlamydomonas reinhardtii, respectively, while some of the mapped ends were not described previously and␣might therefore represent additional origins of replication. Springer Science+Business Media B.V., 2006 Replication nationallicence Chloroplast nationallicence PFGE nationallicence Tobacco nationallicence Origin of replication nationallicence Plastome nationallicence IR : Inverted repeat in the plastome nationallicence LSC : Large single copy region in the plastome nationallicence ORI : Origin of replication nationallicence PFGE : Pulsed-field gel electrophoresis nationallicence ptDNA : Plastid DNA nationallicence SSC : Small single copy region in the plastome nationallicence Scharff Lars Department of Biology I - Botany, University of Munich, Menzinger Str. 67, D-80638, München, Germany aut Koop Hans-Ulrich Department of Biology I - Botany, University of Munich, Menzinger Str. 67, D-80638, München, Germany aut Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 611-621 0167-4412 62:4-5<611 2006 62 11103 https://doi.org/10.1007/s11103-006-9042-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9042-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Scharff Lars Department of Biology I - Botany, University of Munich, Menzinger Str. 67, D-80638, München, Germany aut NATIONALLICENCE 700 1- Koop Hans-Ulrich Department of Biology I - Botany, University of Munich, Menzinger Str. 67, D-80638, München, Germany aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 611-621 0167-4412 62:4-5<611 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer