caa a22 4500 467894760 CHVBK 20180406152759.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s11103-006-9055-5 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9055-5 A novel wound-responsive cis -element, VWRE, of the vascular system-specific expression of a tobacco peroxidase gene, tpoxN1 [Elektronische Daten] [Katsutomo Sasaki, Hiroyuki Ito, Ichiro Mitsuhara, Susumu Hiraga, Shigemi Seo, Hirokazu Matsui, Yuko Ohashi] The wound-induced expression of tpoxN1, encoding a tobacco peroxidase, is unique because of its vascular system-specific expression and insensitivity to known wound-signal compounds such as jasmonic acid, ethylene, and plant hormones [Sasaki etal. (2002) Plant Cell Physiol 43:108-117]. To study the mechanism of expression, the 2-kbp tpoxN1 promoter region and successive 5′-deletion of the promoter were introduced as GUS fusion genes into tobacco plants. Analysis of GUS activity in transgenic plants indicated that a vascular system-specific and wound-responsive cis-element (VWRE) is present at the −239/−200 region of the promoter. Gel mobility shift assays suggested that a nuclear factor(s) prepared from wounded tobacco stems binds a 14-bp sequence (−229/−215) in the −239/−200 region in a sequence-specific manner. A mutation in this 14-bp region of the −239 promoter fragment resulted in a considerable decrease in wound-responsive GUS activity in transgenic plants. An 11-bp sequence, which completely overlaps with the 14-bp sequence, was found in the 5′ distal region (−420/−410) and is thought to contribute to the wound-induced expression together with the 14-bp. The −114-bp core promoter of the tpoxN1 gene was indispensable for wound-induced expression, indicating that the 14-bp region is a novel wound-responsive cis-element VWRE, which may work cooperatively with other factors in the promoter. Springer Science+Business Media B.V., 2006 Wounding nationallicence Tobacco peroxidase nationallicence Promoter analysis nationallicence cis -element nationallicence Ethylene-independent nationallicence Jasmonic acid-independent nationallicence Sasaki Katsutomo Division of Plant Sciences, Organization of National Institute of Agrobiological Sciences, 305-8602, Tsukuba, Ibaraki, Japan aut Ito Hiroyuki Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan aut Mitsuhara Ichiro Division of Plant Sciences, Organization of National Institute of Agrobiological Sciences, 305-8602, Tsukuba, Ibaraki, Japan aut Hiraga Susumu National Institute of Crop Science (NICS), National Agriculture and Food Research Organization (NARO), 305-8518, Tsukuba, Ibaraki, Japan aut Seo Shigemi Division of Plant Sciences, Organization of National Institute of Agrobiological Sciences, 305-8602, Tsukuba, Ibaraki, Japan aut Matsui Hirokazu Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan aut Ohashi Yuko Division of Plant Sciences, Organization of National Institute of Agrobiological Sciences, 305-8602, Tsukuba, Ibaraki, Japan aut Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 753-768 0167-4412 62:4-5<753 2006 62 11103 https://doi.org/10.1007/s11103-006-9055-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9055-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sasaki Katsutomo Division of Plant Sciences, Organization of National Institute of Agrobiological Sciences, 305-8602, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Ito Hiroyuki Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan aut NATIONALLICENCE 700 1- Mitsuhara Ichiro Division of Plant Sciences, Organization of National Institute of Agrobiological Sciences, 305-8602, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Hiraga Susumu National Institute of Crop Science (NICS), National Agriculture and Food Research Organization (NARO), 305-8518, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Seo Shigemi Division of Plant Sciences, Organization of National Institute of Agrobiological Sciences, 305-8602, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Matsui Hirokazu Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan aut NATIONALLICENCE 700 1- Ohashi Yuko Division of Plant Sciences, Organization of National Institute of Agrobiological Sciences, 305-8602, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 753-768 0167-4412 62:4-5<753 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer