caa a22 4500 467894779 CHVBK 20180406152759.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s11103-006-9036-8 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9036-8 Histone H3 variants in male gametic cells of lily and H3 methylation in mature pollen [Elektronische Daten] [Takashi Okada, Mohan Singh, Prem Bhalla] Histones are vital structural proteins of chromatin that influence its dynamics and function. The tissue-specific expression of histone variants has been shown to regulate the expression of specific genes and genomic stability in animal systems. Here we report on the characterization of five histone H3 variants expressed in Lilium generative cell. The gcH3 and leH3 variants show unique sequence diversity by lacking a conserved lysine residue at position 9 (H3K9). The gH3 shares conserved structural features with centromeric H3 of Arabidopsis. The gH3 variant gene is strongly expressed in generative cells and gH3 histone is incorporated in to generative cell chromatin. The lysine residue of H3 at position 4 (H3K4) is highly methylated in the nuclei of generative cells of mature pollen, while methylation of H3K4 is low in vegetative cell nuclei. Taken together, these results suggest that male gametic cells of Lilium have unique chromatin state and histone H3 variants and their methylation might be involved in gene regulation of male gametic cells. Springer Science+Business Media B.V., 2006 Generative cell nationallicence Histone H3 nationallicence Lilium longiflorum nationallicence Male gamete nationallicence Histone variants nationallicence Centromeric histone H3 nationallicence CenH3 : centromeric histone H3 nationallicence EST : expressed sequence tag nationallicence GC : generative cell nationallicence gcH3 : a generative cell histone variant reported by Xu et al. (1999a) nationallicence gH3 : a generative cell histone variant reported by Ueda et al. (2000) nationallicence H3K4 : Lys 4 residue of histone H3 nationallicence H3K4Me2 : H3K4 dimethylation nationallicence H3K9 : Lys 9 residue of histone H3 nationallicence H3K9Me2 : H3K9 dimethylation nationallicence DAPI : 4',6-diamidino-2-phenylindole nationallicence Okada Takashi Plant Molecular Biology and Biotechnology laboratory, Australian Research Council Centre of Excellence for Integrative Legume Research, Faculty of Land and Food Resources, The University of Melbourne, 3010, Parkville, Victoria, Australia aut Singh Mohan Plant Molecular Biology and Biotechnology laboratory, Australian Research Council Centre of Excellence for Integrative Legume Research, Faculty of Land and Food Resources, The University of Melbourne, 3010, Parkville, Victoria, Australia aut Bhalla Prem Plant Molecular Biology and Biotechnology laboratory, Australian Research Council Centre of Excellence for Integrative Legume Research, Faculty of Land and Food Resources, The University of Melbourne, 3010, Parkville, Victoria, Australia aut Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 503-512 0167-4412 62:4-5<503 2006 62 11103 https://doi.org/10.1007/s11103-006-9036-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9036-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Okada Takashi Plant Molecular Biology and Biotechnology laboratory, Australian Research Council Centre of Excellence for Integrative Legume Research, Faculty of Land and Food Resources, The University of Melbourne, 3010, Parkville, Victoria, Australia aut NATIONALLICENCE 700 1- Singh Mohan Plant Molecular Biology and Biotechnology laboratory, Australian Research Council Centre of Excellence for Integrative Legume Research, Faculty of Land and Food Resources, The University of Melbourne, 3010, Parkville, Victoria, Australia aut NATIONALLICENCE 700 1- Bhalla Prem Plant Molecular Biology and Biotechnology laboratory, Australian Research Council Centre of Excellence for Integrative Legume Research, Faculty of Land and Food Resources, The University of Melbourne, 3010, Parkville, Victoria, Australia aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 503-512 0167-4412 62:4-5<503 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer