caa a22 4500 467894817 CHVBK 20180406152800.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s11103-006-9054-6 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9054-6 A novel approach for developing resistance in rice against phloem limited viruses by antagonizing the phloem feeding hemipteran vectors [Elektronische Daten] [Prasenjit Saha, Indranil Dasgupta, Sampa Das] Rice production is known to be severely affected by virus transmitting rice pests, brown planthopper (BPH) and green leafhopper (GLH) of the order hemiptera, feeding by phloem abstraction. ASAL, a novel lectin from leaves of garlic (Allium sativum) was previously demonstrated to be toxic towards hemipteran pests when administered in artificial diet as well as in ASAL expressing transgenic plants. In this report ASAL was targeted under the control of phloem-specific Agrobacterium rolC and rice sucrose synthase-1 (RSs1) promoters at the insect feeding site into popular rice cultivar, susceptible to hemipteran pests. PCR, Southern blot and C-PRINS analyses of transgenic plants have confirmed stable T-DNA integration and the transgenes were co-segregated among self-fertilized progenies. The T0 and T1 plants, harbouring single copy of intact T-DNA expression cassette, exhibit stable expression of ASAL in northern and western blot analyses. ELISA showed that the level of expressed ASAL was as high as 1.01% of total soluble protein. Immunohistofluorescence localization of ASAL depicted the expected expression patterns regulated by each promoter type. In-planta bioassay studies revealed that transgenic ASAL adversely affect survival, growth and population of BPH and GLH. GLH resistant T1 plants were further evaluated for the incidence of tungro disease, caused by co-infection of GLH vectored Rice tungro bacilliform virus (RTBV) and Rice tungro spherical virus (RTSV), which appeared to be dramatically reduced. The result presented here is the first report of such GLH mediated resistance to infection by RTBV/RTSV in ASAL expressing transgenic rice plant. Springer Science+Business Media B.V., 2006 ASAL transgenic rice nationallicence GLH nationallicence Immunohistofluorescence nationallicence RTBV resistance nationallicence ASAL : Allium sativum agglutinin from leaf nationallicence BAP : benzylamino purine nationallicence BPH : brown planthopper nationallicence BSA : bovine serum albumin nationallicence cv. : cultivar nationallicence C-PRINS : cycling-primed in situ labelling nationallicence dpi : days post inoculation nationallicence ELISA : enzyme linked immunosorbent assay nationallicence FITC : fluorescein isothiocyanate nationallicence GLH : green leafhopper nationallicence Hyg : hygromycin nationallicence MS : Murashige and Skoog nationallicence NAA : α-napthaline acetic acid nationallicence PBS : phosphate buffered saline nationallicence RSs1 : rice sucrose synthase-1 promoter nationallicence RTBV : Rice tungro bacilliform virus nationallicence RTSV : Rice tungro spherical virus nationallicence SDS-PAGE : sodium dodecyl sulphate-polyacrylamide gel electrophoresis nationallicence Saha Prasenjit Plant Molecular and Cellular Genetics, Bose Institute, P1/12 CIT Scheme VIIM, 700054, Kolkata, India aut Dasgupta Indranil Department of Plant Molecular Biology, University Delhi South Campus, Benito Jaurez Road, 110021, New Delhi, India aut Das Sampa Plant Molecular and Cellular Genetics, Bose Institute, P1/12 CIT Scheme VIIM, 700054, Kolkata, India aut Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 735-752 0167-4412 62:4-5<735 2006 62 11103 https://doi.org/10.1007/s11103-006-9054-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9054-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saha Prasenjit Plant Molecular and Cellular Genetics, Bose Institute, P1/12 CIT Scheme VIIM, 700054, Kolkata, India aut NATIONALLICENCE 700 1- Dasgupta Indranil Department of Plant Molecular Biology, University Delhi South Campus, Benito Jaurez Road, 110021, New Delhi, India aut NATIONALLICENCE 700 1- Das Sampa Plant Molecular and Cellular Genetics, Bose Institute, P1/12 CIT Scheme VIIM, 700054, Kolkata, India aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 735-752 0167-4412 62:4-5<735 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer