caa a22 4500 467894825 CHVBK 20180619060947.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s11103-006-9045-7 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9045-7 Transcriptional changes in powdery mildew infected wheat and Arabidopsis leaves undergoing syringolin-triggered hypersensitive cell death at infection sites [Elektronische Daten] [Kathrin Michel, Olaf Abderhalden, Rémy Bruggmann, Robert Dudler] Blumeria graminis f.sp. tritici, the causal agent of powdery mildew in wheat, is an obligate biotrophic fungus that exclusively invades epidermal cells. As previously shown, spraying of a solution of syringolin A, a circular peptide derivative secreted by the phytopathogenic bacterium Pseudomonas syringae pv. syringae, triggers hypersensitive cell death at infection sites in powdery mildew infected wheat. Thus, the fungus is essentially eradicated. Here we show that syringolin A also triggers hypersensitive cell death in Arabidopsis infected with the powdery mildew fungus Erysiphe cichoracearum. To monitor transcriptional changes associated with this effect, we cloned 307 cDNA clones representing 158 unigenes from powdery mildew infected, syringolin A sprayed wheat leaves by a suppression subtractive hybridization cloning procedure. These cDNAs were microarrayed onto glass slides together with 1088 cDNA-AFLP clones from powdery mildew-infected wheat. Microarray hybridization experiments were performed with probes derived from leaves, epidermal tissue, and mesophyll preparations of mildewed or uninfected wheat plants after syringolin A or control treatment. Similar experiments were performed in Arabidopsis using the Affymetrix ATH1 whole genome GeneChip. The results indicate a conserved mode of action of syringolin A as similar gene groups are induced in both species. Prominent groups include genes associated with the proteasomal degradation pathway, mitochondrial and other heat shock genes, genes involved in mitochondrial alternative electron pathways, and genes encoding glycolytic and fermentative enzymes. Surprisingly, in both species the observed transcriptional response to syringolin A was considerably weaker in infected plants as compared to uninfected plants. The results lead to the working hypothesis that cell death observed at infection sites may result from a parasite-induced suppression of the transcriptional response and thus to insufficient production of protective proteins necessary for the recovery of these cells from whatever insult is imposed by syringolin A. Springer Science+Business Media B.V., 2006 Blumeria graminis nationallicence Erysiphe cichoracearum nationallicence Hypersensitive response nationallicence Microarray nationallicence Pseudomonas syringae nationallicence Michel Kathrin Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, CH-8000, Zurich, Switzerland aut Abderhalden Olaf Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, CH-8000, Zurich, Switzerland aut Bruggmann Rémy Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, CH-8000, Zurich, Switzerland aut Dudler Robert Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, CH-8000, Zurich, Switzerland aut Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 561-578 0167-4412 62:4-5<561 2006 62 11103 https://doi.org/10.1007/s11103-006-9045-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9045-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Michel Kathrin Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, CH-8000, Zurich, Switzerland aut NATIONALLICENCE 700 1- Abderhalden Olaf Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, CH-8000, Zurich, Switzerland aut NATIONALLICENCE 700 1- Bruggmann Rémy Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, CH-8000, Zurich, Switzerland aut NATIONALLICENCE 700 1- Dudler Robert Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, CH-8000, Zurich, Switzerland aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 561-578 0167-4412 62:4-5<561 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 467894825 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer