caa a22 4500 467894833 CHVBK 20180406152800.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s11103-006-9047-5 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9047-5 Molecular dissection of the interaction between the transcriptional activator ZmMRP-1 and the promoter of BETL-1 [Elektronische Daten] [Cristina Barrero, Luis Muñiz, Elisa Gómez, Gregorio Hueros, Joaquín Royo] The interaction between the transfer cell specific transcriptional activator ZmMRP-1 and the promoter of the transfer cell specific gene BETL-1 constitutes an exceptionally robust system. Reporter constructs containing the BETL-1 promoter are virtually silent in a variety of cell types, from maize leaves to yeast. The introduction of ZmMRP-1 in co-transformation assays leads to the transactivation of the reporter construct by up to two orders of magnitude. In this work we have investigated the molecular basis of this interaction. We found that the BETL-1 promoter includes four potential targets for ZmMRP-1 binding, consisting of a 12bp motif containing two repeats. Co-transformation assays and electrophoretic mobility shift experiments identified the sequence TATCTCTATCTC as the preferred one for the interaction with the transcription factor. Identification of similar sequences in other transfer cell specific promoters lead us to propose as a transfer cell box a sequence related to those identified in the BETL-1 promoter, positioned 50-100bp upstream the TATA box. Springer Science+Business Media B.V., 2006 Endosperm transfer cells nationallicence Maize nationallicence Transcriptional regulation nationallicence Barrero Cristina Departamento de Biología Celular y Genética, Universidad de Alcalá, E-28871, Alcala de Henares (Madrid), Spain aut Muñiz Luis Departamento de Biología Celular y Genética, Universidad de Alcalá, E-28871, Alcala de Henares (Madrid), Spain aut Gómez Elisa Departamento de Biología Celular y Genética, Universidad de Alcalá, E-28871, Alcala de Henares (Madrid), Spain aut Hueros Gregorio Departamento de Biología Celular y Genética, Universidad de Alcalá, E-28871, Alcala de Henares (Madrid), Spain aut Royo Joaquín Departamento de Biología Celular y Genética, Universidad de Alcalá, E-28871, Alcala de Henares (Madrid), Spain aut Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 655-668 0167-4412 62:4-5<655 2006 62 11103 https://doi.org/10.1007/s11103-006-9047-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9047-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Barrero Cristina Departamento de Biología Celular y Genética, Universidad de Alcalá, E-28871, Alcala de Henares (Madrid), Spain aut NATIONALLICENCE 700 1- Muñiz Luis Departamento de Biología Celular y Genética, Universidad de Alcalá, E-28871, Alcala de Henares (Madrid), Spain aut NATIONALLICENCE 700 1- Gómez Elisa Departamento de Biología Celular y Genética, Universidad de Alcalá, E-28871, Alcala de Henares (Madrid), Spain aut NATIONALLICENCE 700 1- Hueros Gregorio Departamento de Biología Celular y Genética, Universidad de Alcalá, E-28871, Alcala de Henares (Madrid), Spain aut NATIONALLICENCE 700 1- Royo Joaquín Departamento de Biología Celular y Genética, Universidad de Alcalá, E-28871, Alcala de Henares (Madrid), Spain aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 655-668 0167-4412 62:4-5<655 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer