caa a22 4500 46789485X CHVBK 20180406152800.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s11103-006-9043-9 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9043-9 Two new grape cultivars, bud sports of Cabernet Sauvignon bearing pale-coloured berries, are the result of deletion of two regulatory genes of the berry colour locus [Elektronische Daten] [Amanda Walker, Elizabeth Lee, Simon Robinson] Bud sports are infrequent changes in phenotype affecting shoots of woody perennials but the molecular basis of these mutations has rarely been identified. In this report, we show that the bronze-coloured berries of the Malian cultivar, a documented bud sport of the wine grape Cabernet Sauvignon (Vitis vinifera L.), lack anthocyanins in the subepidermal cells compared to the red/black berried Cabernet Sauvignon in which both the epidermis and several subepidermal cell layers contain anthocyanin. The Malian phenotype is correlated with an alteration in the genome indicated by a reduction of hybridisation signal using a MYBA probe. In Shalistin, a white-berried bud sport of Malian, the red allele at the berry colour locus appears to have been deleted completely. These data suggest that Malian could be a L1/L2 periclinal chimera, which gave rise to Shalistin by an invasion of epidermal cells (L1) by the mutated subepidermal cells (L2). The red grape Pinot Noir has given rise to a number of pale coloured sports, although the provenance of the extant sports is not known. We show that a clone of Pinot Blanc (white-berried) does not have a deletion of the red allele of the same dimensions as that in Shalistin, though a small deletion is a likely explanation for the altered phenotype. However, the mechanism of deletion of the red allele of the berry colour locus is a possible means by which other red to white clonal mutations of grapevines have occurred. Springer Science+Business Media B.V., 2006 Anthocyanin nationallicence Bud sports nationallicence Genetic instability nationallicence Periclinal chimera nationallicence Wine grapes nationallicence Walker Amanda CSIRO Plant Industry, Adelaide Laboratory, PO Box 350, 5064, Glen Osmond, SA, Australia aut Lee Elizabeth CSIRO Plant Industry, Adelaide Laboratory, PO Box 350, 5064, Glen Osmond, SA, Australia aut Robinson Simon CSIRO Plant Industry, Adelaide Laboratory, PO Box 350, 5064, Glen Osmond, SA, Australia aut Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 623-635 0167-4412 62:4-5<623 2006 62 11103 https://doi.org/10.1007/s11103-006-9043-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9043-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Walker Amanda CSIRO Plant Industry, Adelaide Laboratory, PO Box 350, 5064, Glen Osmond, SA, Australia aut NATIONALLICENCE 700 1- Lee Elizabeth CSIRO Plant Industry, Adelaide Laboratory, PO Box 350, 5064, Glen Osmond, SA, Australia aut NATIONALLICENCE 700 1- Robinson Simon CSIRO Plant Industry, Adelaide Laboratory, PO Box 350, 5064, Glen Osmond, SA, Australia aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 623-635 0167-4412 62:4-5<623 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer