caa a22 4500 467894876 CHVBK 20180406152800.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s11103-006-9048-4 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9048-4 Single amino acid alterations in Arabidopsis thaliana RCY1 compromise resistance to Cucumber mosaic virus , but differentially suppress hypersensitive response-like cell death [Elektronische Daten] [Ken-Taro Sekine, Takeaki Ishihara, Shu Hase, Tomonobu Kusano, Jyoti Shah, Hideki Takahashi] Resistance to an yellow strain of Cucumber mosaic virus [CMV(Y)] in Arabidopsis thaliana ecotype C24 is conferred by the CC-NBS-LRR type R gene, RCY1. RCY1-conferred resistance is accompanied by a hypersensitive response (HR), which is characterized by the development of necrotic local lesion (NLL) at the site of infection that restricts viral spread. To further characterize the role of RCY1 in NLL formation we have identified six recessive CMV(Y)-susceptible rcy1 mutants, four of which contain single amino acid substitutions in RCY1: rcy1-2 contains a D to N substitution in the CC domain, rcy1-3 and rcy1-4 contain R to K and E to K substitutions, respectively, in the LRR domain, and rcy1-6 contains a W to C substitution in the NBS domain. The rcy1-5 and rcy1-7 contain nonsense mutations in the LRR and NBS domains, respectively. Although the virus systemically spread in all six rcy1 mutants, HR-associated cell death was differentially induced in these mutants. In comparison to the wild type C24 plant, HR was not observed in the CMV(Y)-inoculated leaves of the rcy1-3, rcy1-5, rcy1-6 and rcy1-7 mutants. In contrast, delayed NLL development was observed in the virus inoculated leaves of the rcy1-2 and rcy1-4 mutants. In addition, necrosis accompanied by elevated accumulation of PR gene transcript also appeared in the non-inoculated leaves of the rcy1-2 and rcy1-4 mutants. Trans-complementation was observed between the rcy1-2 and rcy1-4 alleles; in F1 plants derived from a cross between rcy1-2 and rcy1−4, HR associated cell death was accelerated and systemic spread of the virus was partially suppressed than in the homozygous rcy1-2 and rcy1-4 plants. Our results suggest that the CC, NBS and LRR domains of RCY1 are required for restriction of virus spread but differentially impact the induction of HR-like cell death. Furthermore, these results also predict inter-molecular interaction involving RCY1 in Arabidopsis resistance to CMV(Y). Springer Science+Business Media B.V., 2006 Resistance gene nationallicence Gene-for-gene interaction nationallicence Viral resistance nationallicence CC : Coiled-coil nationallicence CMV(Y) : An yellow strain of Cucumber mosaic virus nationallicence EMS : Ethyl methanesulfonate nationallicence NBS : Nucleotide-binding site nationallicence NLL : Necrotic local lesion nationallicence LRR : Leucine-rich repeats nationallicence RCY1 : RESISTANCE TO CMV(Y) 1 nationallicence TIR : Toll-interleukin-1 receptor nationallicence Sekine Ken-Taro Department of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Miyagi, Sendai, Japan aut Ishihara Takeaki Department of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Miyagi, Sendai, Japan aut Hase Shu Department of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Miyagi, Sendai, Japan aut Kusano Tomonobu Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, 980-8577, Miyagi, Sendai, Japan aut Shah Jyoti Division of Biology and the Molecular, Cellular and Developmental Biology Program,, Kansas State University, 303 Ackert Hall, 66506-4901, Manhattan, KS, U.S.A aut Takahashi Hideki Department of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Miyagi, Sendai, Japan aut Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 669-682 0167-4412 62:4-5<669 2006 62 11103 https://doi.org/10.1007/s11103-006-9048-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9048-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sekine Ken-Taro Department of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Miyagi, Sendai, Japan aut NATIONALLICENCE 700 1- Ishihara Takeaki Department of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Miyagi, Sendai, Japan aut NATIONALLICENCE 700 1- Hase Shu Department of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Miyagi, Sendai, Japan aut NATIONALLICENCE 700 1- Kusano Tomonobu Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, 980-8577, Miyagi, Sendai, Japan aut NATIONALLICENCE 700 1- Shah Jyoti Division of Biology and the Molecular, Cellular and Developmental Biology Program,, Kansas State University, 303 Ackert Hall, 66506-4901, Manhattan, KS, U.S.A aut NATIONALLICENCE 700 1- Takahashi Hideki Department of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Miyagi, Sendai, Japan aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 669-682 0167-4412 62:4-5<669 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer