caa a22 4500 467894884 CHVBK 20180406152800.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s11103-006-9039-5 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9039-5 DEA 1, a circadian- and cold-regulated tomato gene, protects yeast cells from freezing death [Elektronische Daten] [Philip Weyman, Zhiqiang Pan, Qin Feng, David Gilchrist, Richard Bostock] Cold and freezing damage to plants can be mitigated by inducible factors during an acclimation period. DEA1 is a circadian-regulated tomato (Solanum lycopersicum) gene with sequence similarity to EARLI1, an Arabidopsis thaliana gene that confers cold protection. To investigate whether DEA1 was responsive to environmental variables such as cold, cold-treated tomatoes were analyzed for DEA1 expression. DEA1 transcript accumulated in response to cold, and the rapidity of the cold-induced transcript accumulation was regulated by the circadian rhythm. To test whether DEA1 could protect cells from freezing damage, we transformed the yeast, Pichia pastoris, with an inducible DEA1 construct. Yeast cells transformed with the gene survived freezing at a significantly higher rate than control strains and a strain expressing the LacZ gene. Transgenic tomato plants over-expressing or knocking down DEA1 transcript levels did not have an altered phenotype with respect to cold- or pathogen-susceptibility relative to control plants. Springer Science+Business Media B.V., 2006 Circadian rhythm nationallicence Cold stress nationallicence Eight-cysteine motif nationallicence Phytophthora infestans nationallicence Pseudomonas syringae pv. tomato nationallicence ROS : Reactive oxygen species nationallicence FAD : Fatty acid desaturase nationallicence ZT : Zeitgeber time nationallicence MMH : Minimal methanol medium with histidine nationallicence BGMY : Buffered glycerol complex medium nationallicence 8CM : Eight cysteine motif nationallicence LD : Long day nationallicence SD : Short day nationallicence DD : Constant darkness nationallicence LL : Constant light nationallicence AA : Arachidonic Acid nationallicence PTGS : Post-transcriptional gene silencing nationallicence Pst : Pseudomonas syringae pathovar tomato nationallicence LHC : Light harvesting complex nationallicence Weyman Philip Department of Plant Pathology, University of California, One Shields Ave, 95616, Davis, CA, USA aut Pan Zhiqiang Center for Engineering Plants for Resistance Against Pathogens, University of California, One Shields Ave., 95616, Davis, CA, USA aut Feng Qin Department of Plant Pathology, University of California, One Shields Ave, 95616, Davis, CA, USA aut Gilchrist David Department of Plant Pathology, University of California, One Shields Ave, 95616, Davis, CA, USA aut Bostock Richard Department of Plant Pathology, University of California, One Shields Ave, 95616, Davis, CA, USA aut Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 547-559 0167-4412 62:4-5<547 2006 62 11103 https://doi.org/10.1007/s11103-006-9039-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9039-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Weyman Philip Department of Plant Pathology, University of California, One Shields Ave, 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Pan Zhiqiang Center for Engineering Plants for Resistance Against Pathogens, University of California, One Shields Ave., 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Feng Qin Department of Plant Pathology, University of California, One Shields Ave, 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Gilchrist David Department of Plant Pathology, University of California, One Shields Ave, 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Bostock Richard Department of Plant Pathology, University of California, One Shields Ave, 95616, Davis, CA, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 547-559 0167-4412 62:4-5<547 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer