caa a22 4500 467894914 CHVBK 20180406152800.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s11103-006-9051-9 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9051-9 Enhanced flux through the methylerythritol 4-phosphate pathway in Arabidopsis plants overexpressing deoxyxylulose 5-phosphate reductoisomerase [Elektronische Daten] [Lorenzo Carretero-Paulet, Albert Cairó, Patricia Botella-Pavía, Oscar Besumbes, Narciso Campos, Albert Boronat, Manuel Rodríguez-Concepción] The methylerythritol 4-phosphate (MEP) pathway synthesizes the precursors for an astonishing diversity of plastid isoprenoids, including the major photosynthetic pigments chlorophylls and carotenoids. Since the identification of the first two enzymes of the pathway, deoxyxylulose 5-phoshate (DXP) synthase (DXS) and DXP reductoisomerase (DXR), they both were proposed as potential control points. Increased DXS activity has been shown to up-regulate the production of plastid isoprenoids in all systems tested, but the relative contribution of DXR to the supply of isoprenoid precursors is less clear. In this work, we have generated transgenic Arabidopsis thaliana plants with altered DXS and DXR enzyme levels, as estimated from their resistance to clomazone and fosmidomycin, respectively. The down-regulation of DXR resulted in variegation, reduced pigmentation and defects in chloroplast development, whereas DXR-overexpressing lines showed an increased accumulation of MEP- derived plastid isoprenoids such as chlorophylls, carotenoids, and taxadiene in transgenic plants engineered to produce this non-native isoprenoid. Changes in DXR levels in transgenic plants did not result in changes in␣DXS gene expression or enzyme accumulation, confirming that the observed effects on plastid isoprenoid levels in DXR-overexpressing lines were not an indirect consequence of altering DXS levels. The results indicate that the biosynthesis of MEP (the first committed intermediate of the pathway) limits the production of downstream isoprenoids in Arabidopsis chloroplasts, supporting a role for DXR in the control of the metabolic flux through the MEP pathway. Springer Science+Business Media B.V., 2006 Arabidopsis nationallicence Carotenoids nationallicence Deoxyxylulose 5-phosphate reductoisomerase (DXR) nationallicence Isoprenoid biosynthesis nationallicence Methylerythritol 4-phosphate (MEP) pathway nationallicence Taxadiene nationallicence Carretero-Paulet Lorenzo Departament de Bioquímica i Biologia Molecular, Facultat de Biología, Universitat de Barcelona, Av Diagonal 645, 08028, Barcelona, Spain aut Cairó Albert Departament de Bioquímica i Biologia Molecular, Facultat de Biología, Universitat de Barcelona, Av Diagonal 645, 08028, Barcelona, Spain aut Botella-Pavía Patricia Departament de Bioquímica i Biologia Molecular, Facultat de Biología, Universitat de Barcelona, Av Diagonal 645, 08028, Barcelona, Spain aut Besumbes Oscar Departament de Bioquímica i Biologia Molecular, Facultat de Biología, Universitat de Barcelona, Av Diagonal 645, 08028, Barcelona, Spain aut Campos Narciso Departament de Bioquímica i Biologia Molecular, Facultat de Biología, Universitat de Barcelona, Av Diagonal 645, 08028, Barcelona, Spain aut Boronat Albert Departament de Bioquímica i Biologia Molecular, Facultat de Biología, Universitat de Barcelona, Av Diagonal 645, 08028, Barcelona, Spain aut Rodríguez-Concepción Manuel Departament de Bioquímica i Biologia Molecular, Facultat de Biología, Universitat de Barcelona, Av Diagonal 645, 08028, Barcelona, Spain aut Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 683-695 0167-4412 62:4-5<683 2006 62 11103 https://doi.org/10.1007/s11103-006-9051-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9051-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Carretero-Paulet Lorenzo Departament de Bioquímica i Biologia Molecular, Facultat de Biología, Universitat de Barcelona, Av Diagonal 645, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Cairó Albert Departament de Bioquímica i Biologia Molecular, Facultat de Biología, Universitat de Barcelona, Av Diagonal 645, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Botella-Pavía Patricia Departament de Bioquímica i Biologia Molecular, Facultat de Biología, Universitat de Barcelona, Av Diagonal 645, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Besumbes Oscar Departament de Bioquímica i Biologia Molecular, Facultat de Biología, Universitat de Barcelona, Av Diagonal 645, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Campos Narciso Departament de Bioquímica i Biologia Molecular, Facultat de Biología, Universitat de Barcelona, Av Diagonal 645, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Boronat Albert Departament de Bioquímica i Biologia Molecular, Facultat de Biología, Universitat de Barcelona, Av Diagonal 645, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Rodríguez-Concepción Manuel Departament de Bioquímica i Biologia Molecular, Facultat de Biología, Universitat de Barcelona, Av Diagonal 645, 08028, Barcelona, Spain aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 683-695 0167-4412 62:4-5<683 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer