caa a22 4500 467894922 CHVBK 20180406152800.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s11103-006-9049-3 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9049-3 Comparison of maize brown-midrib isogenic lines by cellular UV-microspectrophotometry and comparative transcript profiling [Elektronische Daten] [Chun Shi, Gerald Koch, Milena Ouzunova, Gerhard Wenzel, Imad Zein, Thomas Lübberstedt] The molecular mechanisms underlying cell wall digestibility in maize (Zea mays L.) have been studied in three sets of maize brown-midrib isogenic lines in the genetic background of inbreds 1332 (1332 and 1332 bm3), 5361 (5361 and 5361 bm3), and F2 (F2, F2 bm1, F2 bm2, and F2 bm3). Two complementary approaches, SSH (suppression subtractive hybridization) and microarray-based expression profiling, were used to isolate and identify candidate genes in isogenic lines for bm mutants. Metabolic pathway analysis revealed that transcriptional events caused by altering the expression of a single bm gene involve all metabolic and signaling pathways. 53 ESTs were differentially expressed in all three isogenic bm3 comparisons, whereas 32 ESTs were consistently differentially expressed in different bm isogenic lines in F2 background. About 70% ESTs isolated by SSH were not present on the unigene microarray, demonstrating the usefulness of the SSH procedure to identify genes related to cell wall digestibility. Together with lignin analysis by cellular UV-microspectrophotometry, expression profiling in isogenic bm lines proved to be useful to understand alterations at the sub-cellular and molecular level with respect to lignin composition. The down-regulation of COMT affected the expression of CCoAOMT genes and caused a reduced content both of G and S units in bm3 mutants. Springer Science+Business Media B.V., 2006 Brown-midrib isogenic lines nationallicence Cellular UV-microspectrophotometry nationallicence Silage maize nationallicence Transcript profiling nationallicence Shi Chun Chair of Plant Breeding, Technical University of Munich, Am Hochanger 2, 85350, Freising, Germany aut Koch Gerald Institute for Wood Biology and Wood Protection, Federal Research Centre for Forestry and Forest Products, Leuschnerstrasse 91, 21031, Hamburg, Germany aut Ouzunova Milena KWS Saat AG, Grimsehlstrasse 31, 37555, Einbeck, Germany aut Wenzel Gerhard Chair of Plant Breeding, Technical University of Munich, Am Hochanger 2, 85350, Freising, Germany aut Zein Imad Chair of Plant Breeding, Technical University of Munich, Am Hochanger 2, 85350, Freising, Germany aut Lübberstedt Thomas Department of Genetics and Biotechnology, Research Centre Flakkebjerg, 4200, Slagelse, Denmark aut Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 697-714 0167-4412 62:4-5<697 2006 62 11103 https://doi.org/10.1007/s11103-006-9049-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9049-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shi Chun Chair of Plant Breeding, Technical University of Munich, Am Hochanger 2, 85350, Freising, Germany aut NATIONALLICENCE 700 1- Koch Gerald Institute for Wood Biology and Wood Protection, Federal Research Centre for Forestry and Forest Products, Leuschnerstrasse 91, 21031, Hamburg, Germany aut NATIONALLICENCE 700 1- Ouzunova Milena KWS Saat AG, Grimsehlstrasse 31, 37555, Einbeck, Germany aut NATIONALLICENCE 700 1- Wenzel Gerhard Chair of Plant Breeding, Technical University of Munich, Am Hochanger 2, 85350, Freising, Germany aut NATIONALLICENCE 700 1- Zein Imad Chair of Plant Breeding, Technical University of Munich, Am Hochanger 2, 85350, Freising, Germany aut NATIONALLICENCE 700 1- Lübberstedt Thomas Department of Genetics and Biotechnology, Research Centre Flakkebjerg, 4200, Slagelse, Denmark aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 697-714 0167-4412 62:4-5<697 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer