caa a22 4500 467894930 CHVBK 20180406152800.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s11103-006-9056-4 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9056-4 Molecular and cytogenetic characterization of an Oryza officinalis-O. sativa chromosome 4 addition line and its progenies [Elektronische Daten] [Huajun Jin, Guangxuan Tan, D. Brar, Ming Tang, Gang Li, Lili Zhu, Guangcun He] The wild species Oryza officinalis Wall. ex Watt (2n=24, CC) is a valuable genetic resource for rice (O. sativa L., 2n=24, AA) breeding and genomics research. Genomic in situ hybridization (GISH) and molecular approaches were used to determine the nature and composition of the additional chromosome in a monosomic alien addition line (MAAL) of O. officinalis and its backcross progenies. The extra wild species chromosome in the MAAL (2n=2x=25) was a mosaic one, comprising of the long arm of chromosome 4 from O. officinalis and the short arm from O. sativa. Comparative analysis showed that O. sativa and O. officinalis shared high synteny of restriction fragment length polymorphism (RFLP) markers and low synteny of simple sequence repeat (SSR) markers. A DNA methylation alteration was revealed at C619 in the MAAL and progenies. Analysis of progenies of the MAAL indicated that introgression segments were small in size and introgression was not evenly distributed along the long arm. One recombination hot spot between C513 and RG177 was identified, which is in a gene-rich region. Springer Science+Business Media B.V., 2006 Introgression nationallicence Methylation alteration nationallicence Monosomic alien addition line nationallicence Oryza nationallicence Synteny nationallicence Jin Huajun College of Life Sciences, Wuhan University, Wuchang, 430072, Wuhan, China aut Tan Guangxuan Department of Biology, Zhoukou Normal University, 466001, Zhoukou, China aut Brar D. Genetics and Biochemistry Division, International Rice Research Institute, P.O. Box 933, Manila, Philippines aut Tang Ming College of Life Sciences, Wuhan University, Wuchang, 430072, Wuhan, China aut Li Gang College of Life Sciences, Wuhan University, Wuchang, 430072, Wuhan, China aut Zhu Lili College of Life Sciences, Wuhan University, Wuchang, 430072, Wuhan, China aut He Guangcun College of Life Sciences, Wuhan University, Wuchang, 430072, Wuhan, China aut Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 769-777 0167-4412 62:4-5<769 2006 62 11103 https://doi.org/10.1007/s11103-006-9056-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9056-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jin Huajun College of Life Sciences, Wuhan University, Wuchang, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Tan Guangxuan Department of Biology, Zhoukou Normal University, 466001, Zhoukou, China aut NATIONALLICENCE 700 1- Brar D. Genetics and Biochemistry Division, International Rice Research Institute, P.O. Box 933, Manila, Philippines aut NATIONALLICENCE 700 1- Tang Ming College of Life Sciences, Wuhan University, Wuchang, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Li Gang College of Life Sciences, Wuhan University, Wuchang, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Zhu Lili College of Life Sciences, Wuhan University, Wuchang, 430072, Wuhan, China aut NATIONALLICENCE 700 1- He Guangcun College of Life Sciences, Wuhan University, Wuchang, 430072, Wuhan, China aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/4-5(2006-11-01), 769-777 0167-4412 62:4-5<769 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer