caa a22 4500 467894973 CHVBK 20180406152800.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s11103-006-9062-6 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9062-6 FW2.2 and cell cycle control in developing tomato fruit: a possible example of gene co-option in the evolution of a novel organ [Elektronische Daten] [Bin Cong, Steven Tanksley] fw2.2 is one of the few QTLs thus far isolated from plants and the first one known to control fruit size. While it has been established that FW2.2 is a regulator (either directly or indirectly) of cell division, FW2.2 does not share sequence homology to any protein of known function (Frary etal. Science 289:85-88, 2000; Cong etal. Proc Natl Acad Sci USA 99:13606-13611, 2002; Liu etal. Plant Physiol 132:292-299, 2003). Thus, the mechanism by which FW2.2 mediates cell division in developing fruit is currently unknown. In an effort to remedy this situation, a combination of yeast two-hybrid screens, in vitro binding assays and cell bombardment studies were performed. The results provide strong evidence that FW2.2 physically interacts at or near the plasma membrane with the regulatory (beta) subunit of a CKII kinase. CKII kinases are well-studied in both yeast and animals where they form part of cell cycle related signaling pathway. Thus while FW2.2 is a plant-specific protein and regulates cell division in a specialized plant organ (fruit), it appears to participate in a cell-cycle control signal transduction pathway that predates the divergence of single- and multi-cellular organisms. These results thus provide a glimpse into how ancient and conserved regulatory processes can be co-opted in the evolution of novel organs such as fruit. Springer Science+Business Media B.V., 2006 Tomato fruit nationallicence fw2.2 nationallicence Protein interactions nationallicence CKIIβ nationallicence Gene co-option nationallicence Cong Bin Department of Plant Breeding and Genetics, Department of Plant Biology, Cornell University, Emerson Hall 240, 14853, Ithaca, NY, USA aut Tanksley Steven Department of Plant Breeding and Genetics, Department of Plant Biology, Cornell University, Emerson Hall 240, 14853, Ithaca, NY, USA aut Plant Molecular Biology Kluwer Academic Publishers 62/6(2006-12-01), 867-880 0167-4412 62:6<867 2006 62 11103 https://doi.org/10.1007/s11103-006-9062-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9062-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cong Bin Department of Plant Breeding and Genetics, Department of Plant Biology, Cornell University, Emerson Hall 240, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Tanksley Steven Department of Plant Breeding and Genetics, Department of Plant Biology, Cornell University, Emerson Hall 240, 14853, Ithaca, NY, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/6(2006-12-01), 867-880 0167-4412 62:6<867 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer