caa a22 4500 467894981 CHVBK 20180406152800.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s11103-006-9065-3 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9065-3 A Gateway-based platform for multigene plant transformation [Elektronische Daten] [Qi-Jun Chen, Hai-Meng Zhou, Jia Chen, Xue-Chen Wang] The post-genomic era offers unrivalled opportunities for genetic manipulation of polygenic traits, multiple traits, and multiple gene products. However, remaining technical hurdles make the manipulation of multiple genes in plants difficult. Here we describe a Gateway-based vector system to enable multiple transgenes to be directly linked or fused. The vector system consists of a destination vector and two special attL-flanked entry vectors each containing an attR cassette incompatible with the attL. By multiple rounds of LR recombination reactions, which we call MultiRound Gateway, multiple transgenes can be delivered sequentially and indefinitely into the Gateway-compatible destination vector through alternate use of the two special entry vectors. In our proof-of-principle experiments we have used this vector system to construct a plant transformation vector containing seven functional DNA fragments, including a screening marker gene, two reporter genes and four matrix attachment region sequences. This system provides a platform for fully realizing the potential of plant genetic manipulation. Springer Science+Business Media B.V., 2006 Gateway vectors nationallicence Multigene plant transformation nationallicence MultiRound Gateway nationallicence Chen Qi-Jun State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, 100094, Beijing, China aut Zhou Hai-Meng Department of Biological Science and Biotechnology, Tsinghua University, 100084, Beijing, China aut Chen Jia State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, 100094, Beijing, China aut Wang Xue-Chen State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, 100094, Beijing, China aut Plant Molecular Biology Kluwer Academic Publishers 62/6(2006-12-01), 927-936 0167-4412 62:6<927 2006 62 11103 https://doi.org/10.1007/s11103-006-9065-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9065-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Qi-Jun State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Zhou Hai-Meng Department of Biological Science and Biotechnology, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Chen Jia State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Wang Xue-Chen State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/6(2006-12-01), 927-936 0167-4412 62:6<927 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer