caa a22 4500 467895007 CHVBK 20180406152800.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s11103-006-9064-4 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9064-4 Characterization of the plant Notchless homolog, a WD repeat protein involved in seed development [Elektronische Daten] [Sier-Ching Chantha, B. Emerald, Daniel Matton] We have isolated a plant NOTCHLESS (NLE) homolog from the wild potato species Solanum chacoense Bitt., encoding a WD-repeat containing protein initially characterized as a negative regulator of the Notch receptor in animals. Although no Notch signaling pathway exists in plants, the NLE gene is conserved in animals, plants, and yeast. Overexpression of the plant ScNLE gene in Drosophila similarly affected bristle formation when compared to the overexpression of the endogenous Drosophila NLE gene, suggesting functional conservation. Expression analyses showed that the ScNLE gene was fertilization-induced and primarily expressed in ovules after fertilization, mainly in the integumentary tapetum (endothelium). Significant expression was also detected in the shoot apex. Promoter deletion analysis revealed that the ScNLE promoter had a complex modulatory architecture with both positive, negative, and tissue specific regulatory elements. Transgenic plants with reduced levels of ScNLE transcripts displayed pleitotropic phenotypes including a severe reduction in seed set, consistent with ScNLE gene expression pattern. Springer Science+Business Media B.V., 2006 Notchless nationallicence WD-repeat protein nationallicence Fertilization nationallicence Ovule nationallicence Seed nationallicence DAP : Days after pollination nationallicence HAP : Hours after pollination nationallicence Chantha Sier-Ching Institut de Recherche en Biologie Végétale (IRBV), Département de sciences biologiques, Université de Montréal, 4101 rue Sherbrooke est, H1X 2B2, Montréal, QC, Canada aut Emerald B. The Liggins Institute, Faculty of Medical and Health Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand aut Matton Daniel Institut de Recherche en Biologie Végétale (IRBV), Département de sciences biologiques, Université de Montréal, 4101 rue Sherbrooke est, H1X 2B2, Montréal, QC, Canada aut Plant Molecular Biology Kluwer Academic Publishers 62/6(2006-12-01), 897-912 0167-4412 62:6<897 2006 62 11103 https://doi.org/10.1007/s11103-006-9064-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9064-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chantha Sier-Ching Institut de Recherche en Biologie Végétale (IRBV), Département de sciences biologiques, Université de Montréal, 4101 rue Sherbrooke est, H1X 2B2, Montréal, QC, Canada aut NATIONALLICENCE 700 1- Emerald B. The Liggins Institute, Faculty of Medical and Health Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand aut NATIONALLICENCE 700 1- Matton Daniel Institut de Recherche en Biologie Végétale (IRBV), Département de sciences biologiques, Université de Montréal, 4101 rue Sherbrooke est, H1X 2B2, Montréal, QC, Canada aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/6(2006-12-01), 897-912 0167-4412 62:6<897 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer