caa a22 4500 467895058 CHVBK 20180406152800.0 cr unu---uuuuu 170328e20061201xx s 000 0 eng 10.1007/s11103-006-9057-3 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9057-3 The cationic cell-wall-peroxidase having oxidation ability for polymeric substrate participates in the late stage of lignification of Populus alba L [Elektronische Daten] [Shinya Sasaki, Kei'ichi Baba, Tomoaki Nishida, Yuji Tsutsumi, Ryuichiro Kondo] Previously we reported that purified Cell Wall Peroxidase-Cationic (CWPO-C) from poplar callus (Populus alba L.) oxidizes sinapyl alcohol and polymeric substrate unlike other plant peroxidases and proposed that this isoenzyme is a conceivable lignification specific peroxidase. In this study, we cloned full-length cDNA of CWPO-C and investigated the transcription of CWPO-C gene in various organs and the localization of CWPO-C protein in the differentiating xylem of poplar stem. Real-time PCR analyses indicated that CWPO-C gene is constitutively expressed in the developing xylem, leaf, and shoot but not affected by many stress treatments. Immunohistochemical analysis showed that CWPO-C locates in the middle lamellae, cell corners, and secondary cell walls of the fiber cells during the lignification. The intensity of the CWPO-C labeling increased gradually from the cell wall thickening stage to mature stage of fiber cells, which is very consistent with the increase of lignin content in the developing xylem. These results strongly support that CWPO-C is responsible for the lignification of the secondary xylem. Interestingly, immuno-labeling of CWPO-C was also observed inside of the ray parenchyma cells instead no signals were detected within the developing fiber cells. This suggests that CWPO-C is biosynthesized in the parenchyma cells and provided to the middle lamellae, the cell corners, and the cell walls to achieve lignin polymerization. Springer Science+Business Media B.V., 2006 Lignification nationallicence Peroxidase nationallicence Poplar nationallicence Immunolocalization nationallicence Secondary xylem nationallicence Cell wall nationallicence CWPO-C : Cell wall peroxidase-cationic nationallicence 2,4-D : 2,4-Dichlorophenoxyacetic acid nationallicence Tris : 2-Amino-2-hydroxymethylpropane-1,3-diol nationallicence SDS-PAGE : Sodium dodecyl sulfate polyacrylamidegel-electrophoresis nationallicence PBS : Phosphate buffered saline nationallicence Tween : Poly(oxyethylene) sorbitan monolaulate nationallicence IgG : Immune globulin G nationallicence PVDF : Polyvinylidene difluoride nationallicence PIPES : Piperazine-N,N′-bis(2-ethanesulfonic acid) nationallicence BSA : Albumin from bovine serum nationallicence CTAB : Cetyltrimethyl-aminonium bromide nationallicence cDNA : Complementary DNA nationallicence PCR : Polymerase chain reaction nationallicence DHP : Dehydrogenative polymer nationallicence PAL : Phenylalanine ammonialyase nationallicence 4CL : 4-Coumarate:coenzyme A ligase nationallicence CAD : Cinnamyl alcohol dehydrogenase nationallicence CCoAOMT : Caffeoyl-CoA 3-O-methyltransferase nationallicence CBB : Coomassie brilliant blue R-250 nationallicence RACE : Rapid amplification of cDNA ends nationallicence Sasaki Shinya Department of Forest and Forest Products Sciences, Kyushu University, 6-10-1 Hakozaki, 812-8581, Higashiku, Fukuoka, Japan aut Baba Kei'ichi Research Institute for Sustainable Humanosphere, Kyoto University, 611-0011, Gokasho, Uji, Japan aut Nishida Tomoaki Department of Forest Resource Science, Shizuoka University, 836 Ohya, 422-8529, Shizuoka, Japan aut Tsutsumi Yuji Department of Forest and Forest Products Sciences, Kyushu University, 6-10-1 Hakozaki, 812-8581, Higashiku, Fukuoka, Japan aut Kondo Ryuichiro Department of Forest and Forest Products Sciences, Kyushu University, 6-10-1 Hakozaki, 812-8581, Higashiku, Fukuoka, Japan aut Plant Molecular Biology Kluwer Academic Publishers 62/6(2006-12-01), 797-807 0167-4412 62:6<797 2006 62 11103 https://doi.org/10.1007/s11103-006-9057-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9057-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sasaki Shinya Department of Forest and Forest Products Sciences, Kyushu University, 6-10-1 Hakozaki, 812-8581, Higashiku, Fukuoka, Japan aut NATIONALLICENCE 700 1- Baba Kei'ichi Research Institute for Sustainable Humanosphere, Kyoto University, 611-0011, Gokasho, Uji, Japan aut NATIONALLICENCE 700 1- Nishida Tomoaki Department of Forest Resource Science, Shizuoka University, 836 Ohya, 422-8529, Shizuoka, Japan aut NATIONALLICENCE 700 1- Tsutsumi Yuji Department of Forest and Forest Products Sciences, Kyushu University, 6-10-1 Hakozaki, 812-8581, Higashiku, Fukuoka, Japan aut NATIONALLICENCE 700 1- Kondo Ryuichiro Department of Forest and Forest Products Sciences, Kyushu University, 6-10-1 Hakozaki, 812-8581, Higashiku, Fukuoka, Japan aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/6(2006-12-01), 797-807 0167-4412 62:6<797 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer