caa a22 4500 467895082 CHVBK 20180406152800.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s11103-006-9026-x doi (NATIONALLICENCE)springer-10.1007/s11103-006-9026-x Molecular characterization of three non-functional S -haplotypes in sour cherry ( Prunus cerasus ) [Elektronische Daten] [Tatsuya Tsukamoto, Nathanael Hauck, Ryutaro Tao, Ning Jiang, Amy Iezzoni] Tetraploid sour cherry (Prunus cerasus) exhibits a genotype-dependent loss of gametophytic self-incompatibility that is caused by the accumulation of non-functional S-haplotypes with disrupted pistil component (stylar-S) and/or pollen component (pollen-S) function. Genetic studies using diverse sour cherry germplasm identified non-functional S-haplotypes for which an equivalent wild-type S-haplotype was present in sweet cherry (Prunus avium), a diploid progenitor of sour cherry. In all cases, the non-functional S-haplotype resulted from mutations affecting the stylar component S-RNase or Prunus pollen component S-haplotype-specific F-box protein (SFB). This study determines the molecular bases of three of these S-haplotypes that confer unilateral incompatibility, two stylar-part mutants (S 6m2 and S 13m ) and one pollen-part mutant (S 13 ′). Compared to their wild-type alleles, S 6m2 -RNase has a 1bp deletion, S 13m -RNase has a 23bp deletion and SFB 13 ′ has a 1bp substitution that lead to premature stop codons. Transcripts were identified for these three alleles, S 6m2 -RNase, S 13m -RNase, and SFB 13 ′, however, these transcripts presumably result in altered proteins with a resulting loss of activity. Our characterization of natural pollen-part and stylar-part mutants in sour cherry along with other natural S-haplotype mutants identified in Prunus supports the view that loss of pollen specificity and stylar rejection evolve independently and are caused by structural alterations affecting the S-haplotype. The prevalence of non-functional S-haplotypes in sour cherry but not in sweet cherry (a diploid) suggests that polyploidization and gene duplication were indirectly responsible for the dysfunction of some S-haplotypes and the emergence of self-compatibility in sour cherry. This resembles the specific mode of evolution in yeast where accelerated evolution occurred to one member of the duplicated gene pair. Springer Science+Business Media B.V., 2006 Pollen-part self-compatible mutant nationallicence Prunus nationallicence Self-incompatibility nationallicence S -haplotype-specific F-box protein nationallicence S-RNase nationallicence Stylar-part self-compatible mutant nationallicence GSI : Gametophytic self-incompatibility nationallicence RT-PCR : Reverse transcriptase PCR nationallicence R : Rheinische nationallicence SI : Self-incompatible nationallicence SC : Self-compatible nationallicence SFB : S-haplotype-specific F-box protein nationallicence Tsukamoto Tatsuya Department of Horticulture, Michigan State University, 48824, East Lansing, MI, USA aut Hauck Nathanael Department of Horticulture, Michigan State University, 48824, East Lansing, MI, USA aut Tao Ryutaro Laboratory of Pomology, Graduate School of Agriculture, Kyoto University, 606-8502, Kyoto, Japan aut Jiang Ning Department of Horticulture, Michigan State University, 48824, East Lansing, MI, USA aut Iezzoni Amy Department of Horticulture, Michigan State University, 48824, East Lansing, MI, USA aut Plant Molecular Biology Kluwer Academic Publishers 62/3(2006-10-01), 371-383 0167-4412 62:3<371 2006 62 11103 https://doi.org/10.1007/s11103-006-9026-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9026-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tsukamoto Tatsuya Department of Horticulture, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Hauck Nathanael Department of Horticulture, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Tao Ryutaro Laboratory of Pomology, Graduate School of Agriculture, Kyoto University, 606-8502, Kyoto, Japan aut NATIONALLICENCE 700 1- Jiang Ning Department of Horticulture, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Iezzoni Amy Department of Horticulture, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/3(2006-10-01), 371-383 0167-4412 62:3<371 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer