caa a22 4500 467895090 CHVBK 20180406152801.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s11103-006-9025-y doi (NATIONALLICENCE)springer-10.1007/s11103-006-9025-y EST database for early flower development in California poppy ( Eschscholzia californica Cham., Papaveraceae) tags over 6000 genes from a basal eudicot [Elektronische Daten] [John Carlson, James Leebens-Mack, P. Wall, Laura Zahn, Lukas Mueller, Lena Landherr, Yi Hu, Daniel Ilut, Jennifer Arrington, Stephanie Choirean, Annette Becker, Dawn Field, Steven Tanksley, Hong Ma, Claude dePamphilis] The Floral Genome Project (FGP) selected California poppy (Eschscholzia californica Cham. ssp. Californica) to help identify new florally-expressed genes related to floral diversity in basal eudicots. A large, non-normalized cDNA library was constructed from premeiotic and meiotic floral buds and sequenced to generate a database of 9079 high quality Expressed Sequence Tags (ESTs). These sequences clustered into 5713 unigenes, including 1414 contigs and 4299 singletons. Homologs of genes regulating many aspects of flower development were identified, including those for organ identity and development, cell and tissue differentiation, cell cycle control, and secondary metabolism. Over 5% of the transcriptome consisted of homologs to known floral gene families. Most are the first representatives of their respective gene families in basal eudicots and their conservation suggests they are important for floral development and/or function. App. 10% of the transcripts encoded transcription factors and other regulatory genes, including nine genes from the seven major lineages of the important MADS-box family of developmental regulators. Homologs of alkaloid pathway genes were also recovered, providing opportunities to explore adaptive evolution in secondary products. Furthermore, comparison of the poppy ESTs with the Arabidopsis genome provided support for putative Arabidopsis genes that previously lacked annotation. Finally, over 1800 unique sequences had no observable homology in the public databases. The California poppy EST database and library will help bridge our understanding of flower initiation and development among higher eudicot and monocot model plants and provide new opportunities for comparative analysis of gene families across angiosperm species. Springer Science+Business Media B.V., 2006 EST database nationallicence Flower development nationallicence California poppy nationallicence Basal eudicot nationallicence ABI : Applied Biosystems nationallicence AG : AGAMOUS gene nationallicence AGL : AGAMOUS-like gene nationallicence AP : APETALA gene nationallicence DEF : DEFICIENS gene nationallicence DEPC : diethylpyrocarbonate nationallicence EF-1 -α : Elongation Factor 1-alpha gene nationallicence ESca : Eschscholzia californica nationallicence EST : Expressed Sequence Tag nationallicence FIM : FIMBRIATA gene nationallicence FLO : FLORICAULA gene nationallicence GO : Gene Ontology Consortium nationallicence GLO : GLOBOSA gene nationallicence Ks : rate of synonymous substitutions nationallicence LFY : LEAFY gene nationallicence Mbp : Million base pairs nationallicence MYA : Million years ago nationallicence MYB : myeloblastosis-like gene nationallicence NCBI : National Center for Biotechnology Information nationallicence PCR : polymerase chain reaction nationallicence PGN : Plant Genome Network nationallicence PI : PISTILLATA gene nationallicence PLE : PLENA gene nationallicence rRNA : ribosomal RNA nationallicence RACE : Rapid Amplification of cDNA Ends nationallicence RCA : Rolling Circle Amplification nationallicence RuBisCO : ribulose-1 nationallicence -bisphosphate carboxylase nationallicence ssp., species nationallicence TAIR : The Arabidopsis Information Resource web site nationallicence Carlson John The School of Forest Resources and Huck Institutes for Life Sciences, Pennsylvania State University, 323 Forest Resources Building, 16802, University Park, PA, USA aut Leebens-Mack James Department of Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut Wall P. Department of Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut Zahn Laura Department of Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut Mueller Lukas Department of Plant Breeding, Cornell University, 14853, Ithaca, NY, USA aut Landherr Lena Department of Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut Hu Yi Department of Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut Ilut Daniel Department of Plant Breeding, Cornell University, 14853, Ithaca, NY, USA aut Arrington Jennifer Department of Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut Choirean Stephanie Department of Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut Becker Annette Evolutionary Developmental Genetics Group, University of Bremen, 28359, Bremen, Germany aut Field Dawn Oxford Centre for Ecology and Hydrology, Mansfield Road, OX1 3SR, Oxford, UK aut Tanksley Steven Department of Plant Breeding, Cornell University, 14853, Ithaca, NY, USA aut Ma Hong Department of Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut dePamphilis Claude Department of Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut Plant Molecular Biology Kluwer Academic Publishers 62/3(2006-10-01), 351-369 0167-4412 62:3<351 2006 62 11103 https://doi.org/10.1007/s11103-006-9025-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9025-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Carlson John The School of Forest Resources and Huck Institutes for Life Sciences, Pennsylvania State University, 323 Forest Resources Building, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Leebens-Mack James Department of Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Wall P. Department of Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Zahn Laura Department of Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Mueller Lukas Department of Plant Breeding, Cornell University, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Landherr Lena Department of Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Hu Yi Department of Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Ilut Daniel Department of Plant Breeding, Cornell University, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Arrington Jennifer Department of Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Choirean Stephanie Department of Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Becker Annette Evolutionary Developmental Genetics Group, University of Bremen, 28359, Bremen, Germany aut NATIONALLICENCE 700 1- Field Dawn Oxford Centre for Ecology and Hydrology, Mansfield Road, OX1 3SR, Oxford, UK aut NATIONALLICENCE 700 1- Tanksley Steven Department of Plant Breeding, Cornell University, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Ma Hong Department of Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- dePamphilis Claude Department of Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/3(2006-10-01), 351-369 0167-4412 62:3<351 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer