caa a22 4500 467895112 CHVBK 20180406152801.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s11103-006-9033-y doi (NATIONALLICENCE)springer-10.1007/s11103-006-9033-y Sucrose deficiency delays lycopene accumulation in tomato fruit pericarp discs [Elektronische Daten] [Nadège Télef, Linda Stammitti-Bert, Anne Mortain-Bertrand, Mickaël Maucourt, Jean Carde, Dominique Rolin, Philippe Gallusci] Tomato (Solanum lycopersicum) fruit ripening is characterized by a massive accumulation of carotenoids (mainly lycopene) as chloroplasts change to chromoplasts. To address the question of the role of sugars in controlling carotenoid accumulation, fruit pericarp discs (mature green fruits) were cultured in vitro in the presence of various sucrose concentrations. A significant difference in soluble sugar content was achieved depending on external sucrose availability. Sucrose limitation delayed and reduced lycopene and phytoene accumulation, with no significant effect on other carotenoids. Chlorophyll degradation and starch catabolism were not affected by variations of sucrose availability. The reduction of lycopene synthesis observed in sucrose-limited conditions was mediated through metabolic changes illustrated by reduced hexose accumulation levels. In addition, variations of sucrose availability modulated PSY1 gene expression. Taken together our results suggest that the modulation of carotenoid accumulation by sucrose availability occurs at the metabolic level and involves the differential regulation of genes involved in carotenoid biosynthesis. Springer Science+Business Media B.V., 2006 Tomato fruit nationallicence Isoprenoids nationallicence Carotenoid nationallicence Lycopene nationallicence Sucrose nationallicence Pericarp discs nationallicence Télef Nadège UMR Physiologie et Biotechnologie Végétales, INRA, Université Bordeaux 1, Université Victor Segalen Bordeaux 2, CR INRA de Bordeaux, 71 Avenue Edouard Bourleaux, BP 81, 33883, Villenave d'Ornon Cedex, France aut Stammitti-Bert Linda UMR Physiologie et Biotechnologie Végétales, INRA, Université Bordeaux 1, Université Victor Segalen Bordeaux 2, CR INRA de Bordeaux, 71 Avenue Edouard Bourleaux, BP 81, 33883, Villenave d'Ornon Cedex, France aut Mortain-Bertrand Anne UMR Physiologie et Biotechnologie Végétales, INRA, Université Bordeaux 1, Université Victor Segalen Bordeaux 2, CR INRA de Bordeaux, 71 Avenue Edouard Bourleaux, BP 81, 33883, Villenave d'Ornon Cedex, France aut Maucourt Mickaël UMR Physiologie et Biotechnologie Végétales, INRA, Université Bordeaux 1, Université Victor Segalen Bordeaux 2, CR INRA de Bordeaux, 71 Avenue Edouard Bourleaux, BP 81, 33883, Villenave d'Ornon Cedex, France aut Carde Jean UMR Physiologie et Biotechnologie Végétales, INRA, Université Bordeaux 1, Université Victor Segalen Bordeaux 2, CR INRA de Bordeaux, 71 Avenue Edouard Bourleaux, BP 81, 33883, Villenave d'Ornon Cedex, France aut Rolin Dominique UMR Physiologie et Biotechnologie Végétales, INRA, Université Bordeaux 1, Université Victor Segalen Bordeaux 2, CR INRA de Bordeaux, 71 Avenue Edouard Bourleaux, BP 81, 33883, Villenave d'Ornon Cedex, France aut Gallusci Philippe UMR Physiologie et Biotechnologie Végétales, INRA, Université Bordeaux 1, Université Victor Segalen Bordeaux 2, CR INRA de Bordeaux, 71 Avenue Edouard Bourleaux, BP 81, 33883, Villenave d'Ornon Cedex, France aut Plant Molecular Biology Kluwer Academic Publishers 62/3(2006-10-01), 453-469 0167-4412 62:3<453 2006 62 11103 https://doi.org/10.1007/s11103-006-9033-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9033-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Télef Nadège UMR Physiologie et Biotechnologie Végétales, INRA, Université Bordeaux 1, Université Victor Segalen Bordeaux 2, CR INRA de Bordeaux, 71 Avenue Edouard Bourleaux, BP 81, 33883, Villenave d'Ornon Cedex, France aut NATIONALLICENCE 700 1- Stammitti-Bert Linda UMR Physiologie et Biotechnologie Végétales, INRA, Université Bordeaux 1, Université Victor Segalen Bordeaux 2, CR INRA de Bordeaux, 71 Avenue Edouard Bourleaux, BP 81, 33883, Villenave d'Ornon Cedex, France aut NATIONALLICENCE 700 1- Mortain-Bertrand Anne UMR Physiologie et Biotechnologie Végétales, INRA, Université Bordeaux 1, Université Victor Segalen Bordeaux 2, CR INRA de Bordeaux, 71 Avenue Edouard Bourleaux, BP 81, 33883, Villenave d'Ornon Cedex, France aut NATIONALLICENCE 700 1- Maucourt Mickaël UMR Physiologie et Biotechnologie Végétales, INRA, Université Bordeaux 1, Université Victor Segalen Bordeaux 2, CR INRA de Bordeaux, 71 Avenue Edouard Bourleaux, BP 81, 33883, Villenave d'Ornon Cedex, France aut NATIONALLICENCE 700 1- Carde Jean UMR Physiologie et Biotechnologie Végétales, INRA, Université Bordeaux 1, Université Victor Segalen Bordeaux 2, CR INRA de Bordeaux, 71 Avenue Edouard Bourleaux, BP 81, 33883, Villenave d'Ornon Cedex, France aut NATIONALLICENCE 700 1- Rolin Dominique UMR Physiologie et Biotechnologie Végétales, INRA, Université Bordeaux 1, Université Victor Segalen Bordeaux 2, CR INRA de Bordeaux, 71 Avenue Edouard Bourleaux, BP 81, 33883, Villenave d'Ornon Cedex, France aut NATIONALLICENCE 700 1- Gallusci Philippe UMR Physiologie et Biotechnologie Végétales, INRA, Université Bordeaux 1, Université Victor Segalen Bordeaux 2, CR INRA de Bordeaux, 71 Avenue Edouard Bourleaux, BP 81, 33883, Villenave d'Ornon Cedex, France aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/3(2006-10-01), 453-469 0167-4412 62:3<453 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer