caa a22 4500 467895120 CHVBK 20180406152801.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s11103-006-9027-9 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9027-9 The involvement of chloroplast HSP100/ClpB in the acquired thermotolerance in tomato [Elektronische Daten] [Jin-ying Yang, Ying Sun, Ai-qing Sun, Shu-ying Yi, Jia Qin, Ming-hui Li, Jian Liu] The chloroplast HSP100/ClpB is a newly documented member of the ClpB family, but little was known about its role in imparting thermotolerance to cells. A cDNA coding for a HSP100/ClpB homolog has been cloned from Lycopersicon esculentum and termed as Lehsp100/ClpB (the cDNA sequence of Lehsp100/ClpB has been submitted to the GenBank database under accession number: AB219939). The protein encoded by the cDNA was most similar to the putative chloroplast HSP100/ClpBs in higher plants and the ClpB from Cyanobacterium Synechococcus sp. A 97kDa protein, which matched the predicted size of mature LeHSP100/ClpB, was immunologically detected in chloroplast isolated from heat-treated tomato plants. In addition, the fusion protein, combining the transit sequence of LeHSP100/ClpB and GFP, was found to be located in chloroplast based on the observations of fluorescent microscope images. These results indicated the chloroplast-localization of LeHSP100/ClpB. Both the transcript and the protein of Lehsp100/ClpB were not detected under normal growth conditions, but they were induced by increasingly higher temperatures. An antisense Lehsp100/ClpB cDNA fragment was introduced into the tomato by Agrobacterium-mediated transformation. Antisense lines exhibited an extreme repression of heat-induced expression of Lehsp100/ClpB. The levels of chloroplast HSP60 and small HSP in antisense lines were identical to those of the control plants. After plants preconditioned at 38°C for 2h were exposed to a lethal heat shock at 46°C for 2h, the antisense lines were greatly impaired and withered in 21days of the recovery phase, whereas the untransformed control plants and the vector-transformed plants survived. Furthermore, chlorophyll fluorescence measurements showed that PS II in antisense lines were more susceptible to the thermal irreversible inactivation than the untransformed and vector-transformed control plants. This work provides the first example that induction of chloroplast LeHSP100/ClpB contributes to the acquisition of thermotolerance in higher plants. Springer Science+Business Media B.V., 2006 Acquired thermotolerance nationallicence Chloroplast nationallicence HSP100/ClpB nationallicence Heat shock nationallicence Transgenic tomato nationallicence Yang Jin-ying College of Life Science, Shandong Normal University, Jinan, 250014, Shandong, P.R. China aut Sun Ying College of Life Science, Shandong Normal University, Jinan, 250014, Shandong, P.R. China aut Sun Ai-qing College of Life Science, Shandong Normal University, Jinan, 250014, Shandong, P.R. China aut Yi Shu-ying College of Life Science, Shandong Normal University, Jinan, 250014, Shandong, P.R. China aut Qin Jia College of Life Science, Shandong Normal University, Jinan, 250014, Shandong, P.R. China aut Li Ming-hui College of Life Science, Shandong Normal University, Jinan, 250014, Shandong, P.R. China aut Liu Jian College of Life Science, Shandong Normal University, Jinan, 250014, Shandong, P.R. China aut Plant Molecular Biology Kluwer Academic Publishers 62/3(2006-10-01), 385-395 0167-4412 62:3<385 2006 62 11103 https://doi.org/10.1007/s11103-006-9027-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9027-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Jin-ying College of Life Science, Shandong Normal University, Jinan, 250014, Shandong, P.R. China aut NATIONALLICENCE 700 1- Sun Ying College of Life Science, Shandong Normal University, Jinan, 250014, Shandong, P.R. China aut NATIONALLICENCE 700 1- Sun Ai-qing College of Life Science, Shandong Normal University, Jinan, 250014, Shandong, P.R. China aut NATIONALLICENCE 700 1- Yi Shu-ying College of Life Science, Shandong Normal University, Jinan, 250014, Shandong, P.R. China aut NATIONALLICENCE 700 1- Qin Jia College of Life Science, Shandong Normal University, Jinan, 250014, Shandong, P.R. China aut NATIONALLICENCE 700 1- Li Ming-hui College of Life Science, Shandong Normal University, Jinan, 250014, Shandong, P.R. China aut NATIONALLICENCE 700 1- Liu Jian College of Life Science, Shandong Normal University, Jinan, 250014, Shandong, P.R. China aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/3(2006-10-01), 385-395 0167-4412 62:3<385 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer