caa a22 4500 467895139 CHVBK 20180406152801.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s11103-006-9030-1 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9030-1 The rice OsGAE1 is a novel gibberellin-regulated gene and involved in rice growth [Elektronische Daten] [Asad Jan, Hidemi Kitano, Hiroshi Matsumoto, Setsuko Komatsu] Gibberellins (GAs) are a class of phytohormones that regulate many aspects of plant growth and development processes including stem elongation, flowering, and seed germination. A novel GA-enhanced gene, designated as OsGAE1, was identified using microarray analysis of GA-regulated genes. OsGAE1 expressed in a dose- and time course-dependent manner with minimum expression at 1µM GA3 and maximum expression at 50µM GA3 starting from 30min and peaked at 24h after GA3 treatment. OsGAE1 expression was up-regulated by GA3 at transcript level while no significant effect was observed for other hormones. OsGAE1 was expressed in Escherichia coli with N-terminal His6 tag and the recombinant protein migrated at 38kDa, slightly larger than the predicted 29kDa, during SDS-PAGE. Anti-OsGAE1 antibodies immunoreacted with a protein of 40kDa in rice leaf sheath. OsGAE1 expressed mainly in growing leaf sheath and callus compared to leaf and root. In situ hybridization and OsGAE1 promoter analysis revealed that OsGAE1 expressed in shoot apex meristem and young primary leaves. Northern blot, Western blot, and GUS activities revealed that OsGAE1 is up-regulated by GA3. Transgenic rice expressing OsGAE1 in antisense orientation exhibited severely affected vegetative and reproductive growth. The transgenic plants were 55-70% short compared to control. These results suggest that OsGAE1 is differentially expressed in rice␣leaf sheath in relation to GA3 and it encodes a functional protein which is involved in GA-regulated growth and development of rice. Springer Science+Business Media B.V., 2006 Elongation nationallicence Gene expression nationallicence Gibberellin nationallicence Leaf sheath nationallicence Rice nationallicence Protein expression nationallicence Jan Asad Department of Molecular Biology, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Japan aut Kitano Hidemi Nagoya University, 464-8601, Nagoya, Japan aut Matsumoto Hiroshi Institute of Applied Biochemistry, School of Life and Environmental Sciences, University of Tsukuba, 305-8572, Tsukuba, Japan aut Komatsu Setsuko Department of Molecular Biology, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Japan aut Plant Molecular Biology Kluwer Academic Publishers 62/3(2006-10-01), 439-452 0167-4412 62:3<439 2006 62 11103 https://doi.org/10.1007/s11103-006-9030-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9030-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jan Asad Department of Molecular Biology, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Japan aut NATIONALLICENCE 700 1- Kitano Hidemi Nagoya University, 464-8601, Nagoya, Japan aut NATIONALLICENCE 700 1- Matsumoto Hiroshi Institute of Applied Biochemistry, School of Life and Environmental Sciences, University of Tsukuba, 305-8572, Tsukuba, Japan aut NATIONALLICENCE 700 1- Komatsu Setsuko Department of Molecular Biology, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Japan aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/3(2006-10-01), 439-452 0167-4412 62:3<439 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer