caa a22 4500 467895155 CHVBK 20180406152801.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s11103-006-9034-x doi (NATIONALLICENCE)springer-10.1007/s11103-006-9034-x The Arabidopsis Bio2 protein requires mitochondrial targeting for activity [Elektronische Daten] [Nadège Arnal, Claude Alban, Martine Quadrado, Olivier Grandjean, Hakim Mireau] Mitochondria are involved in the production of various vitamins, such as biotin, in plants. It is unclear why these biosynthetic pathways have been maintained partly or entirely within the mitochondria throughout evolution. The last step in biotin biosynthesis occurs within the mitochondria and is catalyzed by the biotin synthase complex containing the BIO2 gene product. We investigated whether the Arabidopsis Bio2 enzyme could function outside mitochondria, by trying to complement a bio2 mutant with a truncated version of BIO2 lacking the region encoding the mitochondrial targeting sequence. We describe the characterization of a new T-DNA allele of bio2, with the sole phenotype of an absence of biotin production, in contrast to the previously characterized EMS bio2 allele (Patton et al. 1998, Plant Physiol 116(3):935-946). We found that a cytosolic version of the Bio2 protein could not complement this mutant. Supplementation with the substrate dethiobiotin (DTB) also failed to rescue the mutant phenotype. Thus, the lack of availability of DTB in the cytosol is not the only factor preventing this reaction from occurring outside mitochondria. Bio2 requires mitochondrial targeting for activity, enabling it to fulfill its role in biotin synthesis. The reaction catalyzed by Bio2 may be subject to biochemical constraints, and the apparent close connection with the mitochondrial Fe-S machinery may account for the reaction being retained within the organelle. Springer Science+Business Media B.V., 2006 Biotin synthesis nationallicence Mitochondria nationallicence FE-S cluster protein nationallicence Mutant nationallicence Arabidopsis nationallicence Arnal Nadège INRA - Station de Génétique et d'Amélioration des Plantes, Route de Saint-Cyr, F-78026, Versailles cedex, France aut Alban Claude Laboratoire de Physiologie Cellulaire Végétale, CNRS/INRA/Université Joseph Fourier/CEA, 17 rue des martyrs, F-38054, Grenoble cedex 9, France aut Quadrado Martine INRA - Station de Génétique et d'Amélioration des Plantes, Route de Saint-Cyr, F-78026, Versailles cedex, France aut Grandjean Olivier INRA - Station de Génétique et d'Amélioration des Plantes, Route de Saint-Cyr, F-78026, Versailles cedex, France aut Mireau Hakim INRA - Station de Génétique et d'Amélioration des Plantes, Route de Saint-Cyr, F-78026, Versailles cedex, France aut Plant Molecular Biology Kluwer Academic Publishers 62/3(2006-10-01), 471-479 0167-4412 62:3<471 2006 62 11103 https://doi.org/10.1007/s11103-006-9034-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9034-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Arnal Nadège INRA - Station de Génétique et d'Amélioration des Plantes, Route de Saint-Cyr, F-78026, Versailles cedex, France aut NATIONALLICENCE 700 1- Alban Claude Laboratoire de Physiologie Cellulaire Végétale, CNRS/INRA/Université Joseph Fourier/CEA, 17 rue des martyrs, F-38054, Grenoble cedex 9, France aut NATIONALLICENCE 700 1- Quadrado Martine INRA - Station de Génétique et d'Amélioration des Plantes, Route de Saint-Cyr, F-78026, Versailles cedex, France aut NATIONALLICENCE 700 1- Grandjean Olivier INRA - Station de Génétique et d'Amélioration des Plantes, Route de Saint-Cyr, F-78026, Versailles cedex, France aut NATIONALLICENCE 700 1- Mireau Hakim INRA - Station de Génétique et d'Amélioration des Plantes, Route de Saint-Cyr, F-78026, Versailles cedex, France aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/3(2006-10-01), 471-479 0167-4412 62:3<471 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer