caa a22 4500 467895163 CHVBK 20180406152801.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s11103-006-9024-z doi (NATIONALLICENCE)springer-10.1007/s11103-006-9024-z Rice Chlorina-1 and Chlorina-9 encode ChlD and ChlI subunits of Mg-chelatase, a key enzyme for chlorophyll synthesis and chloroplast development [Elektronische Daten] [Haitao Zhang, Jinjie Li, Jeong-Hoon Yoo, Soo-Cheul Yoo, Sung-Hwan Cho, Hee-Jong Koh, Hak Seo, Nam-Chon Paek] Photosynthetic organisms exhibit a green color due to the accumulation of chlorophyll pigments in chloroplasts. Mg-protoporphyrin IX chelatase (Mg-chelatase) comprises three subunits (ChlH, ChlD and ChlI) and catalyzes the insertion of Mg2+ into protoporphyrin IX, the last common intermediate precursor in both chlorophyll and heme biosyntheses, to produce Mg-protoporphyrin IX (MgProto). Chlorophyll deficiency in higher plants results in chlorina (yellowish-green) phenotype. To date, 10 chlorina (chl) mutants have been isolated in rice, but the corresponding genes have not yet been identified. Rice Chl1 and Chl9 genes were mapped to chromosome 3 and isolated by map-based cloning. A missense mutation occurred in a highly conserved amino acid of ChlD in the chl1 mutant and ChlI in the chl9 mutant. Ultrastructural analyses have revealed that the grana are poorly stacked, resulting in the underdevelopment of chloroplasts. In the seedlings fed with aminolevulinate-dipyridyl in darkness, MgProto levels in the chl1 and chl9 mutants decreased up to 25% and 31% of that in wild-type, respectively, indicating that the Mg-chelatase activity is significantly reduced, causing the eventual decrease in chlorophyll synthesis. Furthermore, Northern blot analysis indicated that the nuclear genes encoding the three subunits of Mg-chelatase and LhcpII in chl1 mutant are expressed about 2-fold higher than those in WT, but are not altered in the chl9 mutant. This result indicates that the ChlD subunit participates in negative feedback regulation of plastid-to-nucleus in the expression of nuclear genes encoding chloroplast proteins, but not the ChlI subunit. Springer Science+Business Media B.V., 2006 Rice nationallicence chlorina-1 nationallicence chlorina-9 nationallicence Map-based cloning nationallicence Mg-chelatase nationallicence OsChlD subunit nationallicence OsChlI subunit nationallicence Zhang Haitao Department of Plant Science, Seoul National University, 151-921, Seoul, Republic of Korea aut Li Jinjie Department of Plant Science, Seoul National University, 151-921, Seoul, Republic of Korea aut Yoo Jeong-Hoon Department of Plant Science, Seoul National University, 151-921, Seoul, Republic of Korea aut Yoo Soo-Cheul Department of Plant Science, Seoul National University, 151-921, Seoul, Republic of Korea aut Cho Sung-Hwan Department of Plant Science, Seoul National University, 151-921, Seoul, Republic of Korea aut Koh Hee-Jong Department of Plant Science, Seoul National University, 151-921, Seoul, Republic of Korea aut Seo Hak Department of Plant Science, Seoul National University, 151-921, Seoul, Republic of Korea aut Paek Nam-Chon Department of Plant Science, Seoul National University, 151-921, Seoul, Republic of Korea aut Plant Molecular Biology Kluwer Academic Publishers 62/3(2006-10-01), 325-337 0167-4412 62:3<325 2006 62 11103 https://doi.org/10.1007/s11103-006-9024-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9024-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Haitao Department of Plant Science, Seoul National University, 151-921, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Li Jinjie Department of Plant Science, Seoul National University, 151-921, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Yoo Jeong-Hoon Department of Plant Science, Seoul National University, 151-921, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Yoo Soo-Cheul Department of Plant Science, Seoul National University, 151-921, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Cho Sung-Hwan Department of Plant Science, Seoul National University, 151-921, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Koh Hee-Jong Department of Plant Science, Seoul National University, 151-921, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Seo Hak Department of Plant Science, Seoul National University, 151-921, Seoul, Republic of Korea aut NATIONALLICENCE 700 1- Paek Nam-Chon Department of Plant Science, Seoul National University, 151-921, Seoul, Republic of Korea aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/3(2006-10-01), 325-337 0167-4412 62:3<325 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer