caa a22 4500 467895171 CHVBK 20180406152801.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s11103-006-9031-0 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9031-0 RTS , a rice anther-specific gene is required for male fertility and its promoter sequence directs tissue-specific gene expression in different plant species [Elektronische Daten] [Hong Luo, Jang-Yong Lee, Qian Hu, Kimberly Nelson-Vasilchik, Timothy Eitas, Colin Lickwar, Albert Kausch, Joel Chandlee, Thomas Hodges] A tapetum-specific gene, RTS, has been isolated by differential screening of a cDNA library from rice panicles. RTS is a unique gene in the rice genome. RNA blot analysis and in situ hybridization indicates that this gene is predominantly expressed in the anther's tapetum during meiosis and disappears before anthesis. RTS has no introns and encodes a putative polypeptide of 94amino acids with a hydrophobic N-terminal region. The nucleotide and deduced amino acid sequence of the gene do not show significant homology to any known sequences. However, a sequence in the promoter region, GAATTTGTTA, differs only by one or two nucleotides from one of the conserved motifs in the promoter region of two pollen-specific genes of tomato. Several other sequence motifs found in other anther-specific promoters were also identified in the promoter of the RTS gene. Transgenic and antisense RNA approaches revealed that RTS gene is required for male fertility in rice. The promoter region of RTS, when fused to the Bacillus amyloliquefaciens ribonuclease gene, barnase, or the antisense of the RTS gene, is able to drive tissue-specific expression of both genes in rice, creeping bentgrass (Agrostis stolonifera L.) and Arabidopsis, conferring male sterility to the transgenic plants. Light and near-infrared confocal microscopy of cross-sections through developing flowers of male-sterile transgenics shows that tissue-specific expression of barnase or the antisense RTS genes interrupts tapetal development, resulting in deformed non-viable pollen. These results demonstrate a critical role of the RTS gene in pollen development in rice and the versatile application of the RTS gene promoter in directing anther-specific gene expression in both monocotyledonous and dicotyledonous plants, pointing to a potential for exploiting this gene and its promoter for engineering male sterility for hybrid production of various plant species. Springer Science+Business Media B.V., 2006 RTS nationallicence Tapetum nationallicence Male sterility nationallicence Rice nationallicence Antisense nationallicence barnase nationallicence Luo Hong Department of Genetics, Biochemistry and Life Science Studies, Clemson University, 100 Jordan Hall, 29634, Clemson, SC, USA aut Lee Jang-Yong National Institute of Crop Science, 441-857, Suwon, Korea aut Hu Qian Department of Genetics, Biochemistry and Life Science Studies, Clemson University, 100 Jordan Hall, 29634, Clemson, SC, USA aut Nelson-Vasilchik Kimberly HybriGene, Inc., 530 Liberty Lane, 02892, West Kingston, RI, USA aut Eitas Timothy Department of Cell and Molecular Biology, University of Rhode Island, 02881, Kingston, RI, USA aut Lickwar Colin Department of Cell and Molecular Biology, University of Rhode Island, 02881, Kingston, RI, USA aut Kausch Albert Department of Cell and Molecular Biology, University of Rhode Island, 02881, Kingston, RI, USA aut Chandlee Joel Department of Cell and Molecular Biology, University of Rhode Island, 02881, Kingston, RI, USA aut Hodges Thomas HybriGene, Inc., 530 Liberty Lane, 02892, West Kingston, RI, USA aut Plant Molecular Biology Kluwer Academic Publishers 62/3(2006-10-01), 397-408 0167-4412 62:3<397 2006 62 11103 https://doi.org/10.1007/s11103-006-9031-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9031-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Luo Hong Department of Genetics, Biochemistry and Life Science Studies, Clemson University, 100 Jordan Hall, 29634, Clemson, SC, USA aut NATIONALLICENCE 700 1- Lee Jang-Yong National Institute of Crop Science, 441-857, Suwon, Korea aut NATIONALLICENCE 700 1- Hu Qian Department of Genetics, Biochemistry and Life Science Studies, Clemson University, 100 Jordan Hall, 29634, Clemson, SC, USA aut NATIONALLICENCE 700 1- Nelson-Vasilchik Kimberly HybriGene, Inc., 530 Liberty Lane, 02892, West Kingston, RI, USA aut NATIONALLICENCE 700 1- Eitas Timothy Department of Cell and Molecular Biology, University of Rhode Island, 02881, Kingston, RI, USA aut NATIONALLICENCE 700 1- Lickwar Colin Department of Cell and Molecular Biology, University of Rhode Island, 02881, Kingston, RI, USA aut NATIONALLICENCE 700 1- Kausch Albert Department of Cell and Molecular Biology, University of Rhode Island, 02881, Kingston, RI, USA aut NATIONALLICENCE 700 1- Chandlee Joel Department of Cell and Molecular Biology, University of Rhode Island, 02881, Kingston, RI, USA aut NATIONALLICENCE 700 1- Hodges Thomas HybriGene, Inc., 530 Liberty Lane, 02892, West Kingston, RI, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/3(2006-10-01), 397-408 0167-4412 62:3<397 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer