caa a22 4500 46789518X CHVBK 20180406152801.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s11103-006-9029-7 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9029-7 The ACC synthase TOE sequence is required for interaction with ETO1 family proteins and destabilization of target proteins [Elektronische Daten] [Hitoshi Yoshida, Kevin Wang, Chia-Man Chang, Koichi Mori, Eiji Uchida, Joseph Ecker] The Arabidopsis ETO1 protein is a negative regulator of ethylene biosynthesis. It specifically inhibits the enzyme activity of type 2 1-aminocyclopropane-1-carboxylate synthases (ACC synthases or ACS) and promotes their degradation by a proteasome-dependent pathway. To further understand the function of the ETO1 family in the plant kingdom, we cloned a cDNA of LeEOL1 (Lycopersicon esculentum ETO 1- LIKE 1), an ETO1 homolog from tomato. LeEOL1 encodes a putative protein with domain architecture conserved in the Arabidopsis ETO1/EOL1/EOL2 proteins and in the predicted rice EOL proteins. LeEOL1 is expressed in leaf, stem, root, flower, and the full ripe stage of fruit, suggesting diverse regulatory roles in the development of tomato. Yeast two-hybrid analysis revealed specific interactions between LeEOL1 and type 2 ACC synthases. When the C-terminal 14 amino acids (TOE; target of ETO1) of LE-ACS3 specific to type 2 ACC synthases were fused to a type 1 ACS, LE-ACS2, at the corresponding position, it allowed LE-ACS2 to strongly interact with LeEOL1. A GFP-TOELE-ACS3 fusion protein expressed in rice calli and in the roots of wild-type Arabidopsis showed reduced stability compared to native GFP. However, the fluorescence of GFP-TOELE-ACS3 was comparable to that of the native GFP in Arabidopsis eto1-4 mutant. Furthermore, MG132 treatment significantly enhanced the fluorescence of GFP-TOELE-ACS3 in the roots of wild-type Arabidopsis. These results suggest that the ETO1-family-mediated ACS protein degradation pathway is conserved in both monocots and dicots, and that TOE acts as a protein destabilization signal recognized by the ETO1 protein family. Springer Science+Business Media B.V., 2006 1-Aminocyclopropane-1-carboxylate synthases nationallicence Ethylene biosynthesis nationallicence ETO1 nationallicence Protein degradation nationallicence Protein-protein interaction nationallicence Tomato nationallicence ACC : 1-Aminocyclopropane-1-carboxylate nationallicence ACO : 1-Aminocyclopropane-1-carboxylate oxidase nationallicence ACS : 1-Aminocyclopropane-1-carboxylate synthase nationallicence BTB : Broad-Complex, tramtrack, and bric à brac nationallicence CC : Coiled-coil nationallicence CUL3 : Cullin 3 nationallicence EOL : ETO1-LIKE nationallicence ETO1 : ETHYLENE-OVERPRODUCER 1 nationallicence RT-PCR : Reverse transcription-polymerase chain reaction nationallicence TPR : Tetratricopeptide repeat nationallicence Yoshida Hitoshi Rice Biotechnology Research Subteam (Hokuriku Region), National Agricultural Research Center, 1-2-1, Inada, 943-0193, Jo-etsu, Niigata, Japan aut Wang Kevin Institute of Plant and Microbial Biology, Academia Sinica, 11529, Taipei, Taiwan aut Chang Chia-Man Institute of Plant and Microbial Biology, Academia Sinica, 11529, Taipei, Taiwan aut Mori Koichi Rice Biotechnology Research Subteam (Hokuriku Region), National Agricultural Research Center, 1-2-1, Inada, 943-0193, Jo-etsu, Niigata, Japan aut Uchida Eiji Rice Biotechnology Research Subteam (Hokuriku Region), National Agricultural Research Center, 1-2-1, Inada, 943-0193, Jo-etsu, Niigata, Japan aut Ecker Joseph Plant Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, 92037, La Jolla, CA, USA aut Plant Molecular Biology Kluwer Academic Publishers 62/3(2006-10-01), 427-437 0167-4412 62:3<427 2006 62 11103 https://doi.org/10.1007/s11103-006-9029-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9029-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yoshida Hitoshi Rice Biotechnology Research Subteam (Hokuriku Region), National Agricultural Research Center, 1-2-1, Inada, 943-0193, Jo-etsu, Niigata, Japan aut NATIONALLICENCE 700 1- Wang Kevin Institute of Plant and Microbial Biology, Academia Sinica, 11529, Taipei, Taiwan aut NATIONALLICENCE 700 1- Chang Chia-Man Institute of Plant and Microbial Biology, Academia Sinica, 11529, Taipei, Taiwan aut NATIONALLICENCE 700 1- Mori Koichi Rice Biotechnology Research Subteam (Hokuriku Region), National Agricultural Research Center, 1-2-1, Inada, 943-0193, Jo-etsu, Niigata, Japan aut NATIONALLICENCE 700 1- Uchida Eiji Rice Biotechnology Research Subteam (Hokuriku Region), National Agricultural Research Center, 1-2-1, Inada, 943-0193, Jo-etsu, Niigata, Japan aut NATIONALLICENCE 700 1- Ecker Joseph Plant Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, 92037, La Jolla, CA, USA aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/3(2006-10-01), 427-437 0167-4412 62:3<427 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer