caa a22 4500 467895198 CHVBK 20180406152801.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s11103-006-9021-2 doi (NATIONALLICENCE)springer-10.1007/s11103-006-9021-2 The putative SWI/SNF complex subunit BRAHMA activates flower homeotic genes in Arabidopsis thaliana [Elektronische Daten] [Lidia Hurtado, Sara Farrona, Jose Reyes] Arabidopsis thaliana BRAHMA (BRM, also called AtBRM) is a SNF2 family protein homolog of Brahma, the ATPase of the Drosophila SWI/SNF complex involved in chromatin remodeling during transcription. Here we show that, in contrast to its Drosophila counterpart, BRM is not an essential gene. Thus, homozygous BRM loss of function mutants are viable but exhibit numerous defects including dwarfism, altered leaf and root development and several reproduction defects. The analysis of the progeny of self-fertilized heterozygous brm plants and reciprocal crosses between heterozygous and wild type plants indicated that disruption of BRM reduced both male and female gametophyte transmission. This was consistent with the presence of aborted ovules in the self-fertilized heterozygous flowers that contained arrested embryos predominantly at the two terminal cells stage. Furthermore, brm homozygous mutants were completely sterile. Flowers of brm loss-of-function mutants have several developmental abnormalities, including homeotic transformations in the second and third floral whorls. In accordance with these results, brm mutants present reduced levels of APETALA2, APETALA3, PISTILLATA and NAC-LIKE, ACTIVATED BY AP3/PI. We have previously shown that BRM strongly interacts with AtSWI3C. Now we extend our interaction studies demonstrating that BRM interacts weakly with AtSWI3B but not with AtSWI3A or AtSWI3D. In agreement with these results, the phenotype described in this study for brm plants is very similar to that previously described for the AtSWI3C mutant plants, suggesting that both proteins participate in the same genetic pathway or form a molecular complex. Springer Science+Business Media B.V., 2006 Chromatin nationallicence AtSWI3 nationallicence SWI/SNF complex nationallicence Homeotic gene expression nationallicence Hurtado Lidia Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, Av. Américo Vespucio 49, E-41092, Sevilla, Spain aut Farrona Sara Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, Av. Américo Vespucio 49, E-41092, Sevilla, Spain aut Reyes Jose Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, Av. Américo Vespucio 49, E-41092, Sevilla, Spain aut Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 291-304 0167-4412 62:1-2<291 2006 62 11103 https://doi.org/10.1007/s11103-006-9021-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11103-006-9021-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hurtado Lidia Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, Av. Américo Vespucio 49, E-41092, Sevilla, Spain aut NATIONALLICENCE 700 1- Farrona Sara Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, Av. Américo Vespucio 49, E-41092, Sevilla, Spain aut NATIONALLICENCE 700 1- Reyes Jose Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, Av. Américo Vespucio 49, E-41092, Sevilla, Spain aut NATIONALLICENCE 773 0- Plant Molecular Biology Kluwer Academic Publishers 62/1-2(2006-09-01), 291-304 0167-4412 62:1-2<291 2006 62 11103 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer